Human BTLA PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human BTLA in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BTLA
    Accession # AAP44003
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BTLA in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BTLA PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3354C) or (B) Normal Goat IgG PerCP Control (Catalog # IC108C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BTLA

B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.

  • Long Name:
    B- And T-Lymphocyte Attenuator
  • Entrez Gene IDs:
    151888 (Human); 208154 (Mouse)
  • Alternate Names:
    B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG
PerCP-conjugated Control

Ctrl IC108C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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