Human C-Reactive Protein/CRP DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1707
Ancillary Products Available
Human C-Reactive Protein / CRP ELISA Standard Curve
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Product Details
Procedure
Citations (32)
FAQs
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Reviews (5)

Human C-Reactive Protein/CRP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human C-Reactive Protein/CRP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

  

Data Example

Human C-Reactive Protein / CRP ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human C-Reactive Protein/CRP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Increased Adipose Tissue Expression of Interferon Regulatory Factor (IRF)-5 in Obesity: Association with Metabolic Inflammation
    Authors: S Sindhu, R Thomas, S Kochumon, A Wilson, M Abu-Farha, A Bennakhi, F Al-Mulla, R Ahmad
    Cells, 2019;8(11):.
    Species: Human
    Sample Types: Plasma
  2. Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection
    Authors: TT Tsai, TH Huang, NY Ho, YP Chen, CA Chen, CF Chen
    Sci Rep, 2019;9(1):15679.
    Species: Human
    Sample Types: Synovial Fluid
  3. Prediction and associations of preterm birth and its subtypes with eicosanoid enzymatic pathways and inflammatory markers
    Authors: MT Aung, Y Yu, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, B Mukherjee, JD Meeker
    Sci Rep, 2019;9(1):17049.
    Species: Human
    Sample Types: Plasma
  4. Average and time-specific maternal prenatal inflammatory biomarkers and the risk of labor epidural associated fever
    Authors: DY Arce, A Bellavia, DE Cantonwine, OJ Napoli, JD Meeker, T James-Todd, TF McElrath, LC Tsen
    PLoS ONE, 2019;14(11):e0222958.
    Species: Human
    Sample Types: Plasma
  5. Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children
    Authors: TN Chimponda, C Mushayi, DNM Osakunor, A Vengesai, E Enwono, S Amanfo, J Murray, C Tshuma, F Mutapi, T Mduluza
    BMC Infect. Dis., 2019;19(1):1071.
    Species: Human
    Sample Types: Serum
  6. Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study
    Authors: RE Elphinston, AM Weckman, CR McDonald, V Tran, K Zhong, M Madanitsa, L Kalilani-P, C Khairallah, SM Taylor, SR Meshnick, V Mwapasa, FO Ter Kuile, AL Conroy, KC Kain
    PLoS Med., 2019;16(10):e1002914.
    Species: Human
    Sample Types: Plasma
  7. Systemic inflammation is associated with malaria and preterm birth in women living with HIV on antiretrovirals and co-trimoxazole
    Authors: CR McDonald, AM Weckman, AL Conroy, P Olwoch, P Natureeba, MR Kamya, DV Havlir, G Dorsey, KC Kain
    Sci Rep, 2019;9(1):6758.
    Species: Human
    Sample Types: Plasma
  8. Association of antenatal depression with oxidative stress and impact on spontaneous preterm birth
    Authors: KK Venkatesh, JD Meeker, DE Cantonwine, TF McElrath, KK Ferguson
    J Perinatol, 2019;0(0):.
    Species: Human
    Sample Types: Plasma
  9. Effects of continuous moderate exercise with partial blood flow restriction during hemodialysis: A protocol for a randomized clinical trial
    Authors: RK Cardoso, AM Araujo, RB Orcy, M Bohlke, JP Oses, FB Del Vecchi, FC Barcellos, MC Gonzalez, AJ Rombaldi
    MethodsX, 2019;6(0):190-198.
    Species: Human
    Sample Types: Serum
  10. High serum levels of C-reactive protein (CRP) predict beneficial decrease of visceral fat in obese females after sleeve gastrectomy
    Authors: F Carbone, E Nulli Migl, A Bonaventur, A Vecchié, S De Vuono, MA Ricci, G Vaudo, M Boni, F Dallegri, F Montecucco, G Lupattelli
    Nutr Metab Cardiovasc Dis, 2018;0(0):.
    Species: Human
    Sample Types: Serum
  11. Relationship of the Adherence to a Mediterranean Diet and Its Main Components with CRP Levels in the Spanish Population
    Authors: C Lahoz, E Castillo, JM Mostaza, O de Dios, MA Salinero-F, T González-A, F García-Igl, E Estirado, F Laguna, V Sanchez, C Sabín, S López, V Cornejo, C de Burgos, C Garcés,
    Nutrients, 2018;10(3):.
    Species: Human
    Sample Types: Plasma
  12. Association of calf circumference with insulin resistance and non-alcohol fatty liver disease: the REACTION study
    Authors: W Zhang, Z Yang, Y Niu, X Li, L Zhu, S Lu, H Zhang, J Fan, G Ning, L Qin, Q Su
    BMC Endocr Disord, 2017;17(1):28.
    Species: Human
    Sample Types: Serum
  13. Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways
    Authors: A Burban, A Sharanek, R Hüe, M Gay, S Routier, A Guillouzo, C Guguen-Gui
    Sci Rep, 2017;7(1):1815.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Effects of freezer storage time on levels of complement biomarkers
    Authors: AR Morgan, C O'Hagan, S Touchard, S Lovestone, B Paul Morga
    BMC Res Notes, 2017;10(1):559.
    Species: Human
    Sample Types: Plasma
  15. Improvement of cardiometabolic markers after fish oil intervention in young Mexican adults and the role of PPAR? L162V and PPAR?2 P12A
    Authors: A Binia, C Vargas-Mar, M Ancira-Mor, LM Gosoniu, I Montoliu, E Gámez-Vald, DC Soria-Cont, A Angeles-Qu, R Gonzalez-A, S Fernández, D Martínez-C, B Hernández-, M Ramírez-So, C Pérez-Orte, Y Rodríguez-, I Castan, I Rubio-Alia, F Vadillo-Or, R Márquez-Ve, R Bojalil, JC López-Alva, P Valet, M Kussmann, I Silva-Zole, ME Tejero
    J. Nutr. Biochem., 2017;43(0):98-106.
    Species: Human
    Sample Types: Serum
  16. Experimental type 2 diabetes induction reduces serum vaspin, but not serum omentin, in Wistar rats
    Authors: CA Castro, KA da Silva, MM Buffo, KNZ Pinto, FO Duarte, KO Nonaka, FF Aníbal, ACGO Duarte
    Int J Exp Pathol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  17. Monocyte/macrophage and T cell activation markers are not independently associated with MI risk in healthy individuals - results from the HUNT Study
    Authors: T Ueland, LE Laugsand, LJ Vatten, I Janszky, C Platou, AE Michelsen, JK Damås, P Aukrust, BO Åsvold
    Int. J. Cardiol., 2017;0(0):.
    Species: Human
    Sample Types: Serum
  18. The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis
    Authors: H Lavi, M Assayag, A Schwartz, N Arish, ZG Fridlender, N Berkman
    PLoS ONE, 2017;12(3):e0171945.
    Species: Human
    Sample Types: Plasma
  19. Plasma selenium levels and nonalcoholic fatty liver disease in Chinese adults: a cross-sectional analysis
    Sci Rep, 2016;6(0):37288.
    Species: Human
    Sample Types: Serum
  20. Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects
    Sci Rep, 2016;6(0):37886.
    Species: Human
    Sample Types: Serum
  21. Silk-based blood stabilization for diagnostics
    Proc Natl Acad Sci USA, 2016;113(21):5892-7.
    Species: Human
    Sample Types: Plasma
  22. Intraplaque Expression of C-Reactive Protein Predicts Cardiovascular Events in Patients with Severe Atherosclerotic Carotid Artery Stenosis
    Authors: Aldo Bonaventur
    Mediators Inflamm, 2016;2016(0):9153673.
    Species: Human
    Sample Types: Serum
  23. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  24. Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study.
    Authors: Erdman L, D'Acremont V, Hayford K, Rajwans N, Kilowoko M, Kyungu E, Hongoa P, Alamo L, Streiner D, Genton B, Kain K
    PLoS ONE, 2015;10(9):e0137592.
    Species: Human
    Sample Types: Plasma
  25. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
    Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
    PLoS ONE, 2014;9(2):e89341.
    Species: Human
    Sample Types: Plasma
  26. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
    Species: Human
    Sample Types: Serum
  27. The effect of smoking on DNA methylation of peripheral blood mononuclear cells from African American women.
    Authors: Dogan, Meeshant, Shields, Bridget, Cutrona, Carolyn, Gao, Long, Gibbons, Frederic, Simons, Ronald, Monick, Martha, Brody, Gene H, Tan, Kai, Beach, Steven R, Philibert, Robert A
    BMC Genomics, 2014;15(0):151.
    Species: Human
    Sample Types: Serum
  28. Differences in insulin sensitivity, lipid metabolism and inflammation between young adult Pakistani and Norwegian patients with type 2 diabetes: a cross sectional study.
    Authors: Wium C, Aasheim E, Ueland T, Michelsen A, Thorsby P, Larsen I, Torjesen P, Aukrust P, Birkeland K
    BMC Endocr Disord, 2013;13(0):49.
    Species: Human
    Sample Types: Plasma
  29. Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
    Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
    PLoS ONE, 2011;6(2):e17440.
    Species: Human
    Sample Types: Plasma
  30. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  31. Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers.
    Authors: Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF
    Am. J. Respir. Crit. Care Med., 2009;179(12):1107-14.
    Species: Human
    Sample Types: BALF
  32. Plasma hepcidin is associated with future risk of venous thromboembolism.
    Authors: Ellingsen T, Lappegard J, Ueland T, Aukrust P, Braekkan S, Hansen J
    Blood Adv, 0;2(11):1191-1197.
    Species: Human
    Sample Types: Plasma

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Reviews for Human C-Reactive Protein/CRP DuoSet ELISA

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Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 03/30/2019
Application: Sample Tested: Purified Standard

Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 10/09/2018
Application: Sample Tested: Purified Standard,Urine

Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 09/11/2018
Application: Sample Tested: Purified Standard

Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 12/20/2017
Application: Sample Tested: EDTA Plasma

Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 11/21/2017
Application: Sample Tested: Serum

We used human serum at a dilution of 1:50000. The result was very good.