|Detection of Calcitonin R in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R PE‑conjugated Monoclonal Antibody (Catalog # FAB4614P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Calcitonin Receptor (CALCR) is a glycosylated 55-67 kDa seven-transmembrane G protein-coupled receptor that belongs to the GPCR2 (or Secretin) family of proteins. Alone, CALCR binds Calcitonin, the 32 amino acid (aa) founding member of the 5-member Calcitonin family of peptides. When complexed with RAMP family molecules (RAMP-1, -2, or -3), it serves as a receptor for Amylin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in an isoform showing an alternate start at Met19. The construct used for antibody production starts at Ala25 of the short form or Ala43 of the full-length form. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.