Calsyntenin‑3 in Human Brain. Calsyntenin‑3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Calsyntenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5244) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calsyntenin-3 (CST-3; also CDH-11 and Alcadein-beta ) is a 160 kDa member of the Alcadein family of molecules. It is expressed on the membranes of GABAergic neurons and in the granules of anterior pituitary secretory cells. It is a calcium-binding protein that is proposed to regulate post-synaptic signaling, plus participate in the formation of a membrane-associated APP-X11L-CST-3 complex that blocks APP cleavage. Mature human CST-3 is a 937 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 29-246) in the extracellular region, and an 87 aa cytoplasmic tail. Cleavage presumably occurs, generating a soluble 120 kDa ECD. One splice event shows a 34 aa substitution for the N-terminal 21 amino acids. Over aa 21-849, human CST-3 shares 97% aa identity with mouse CST-3.
Entrez Gene IDs:
9746 (Human); 232370 (Mouse); 171393 (Rat)
alcadein beta; alcadein-beta; alcbeta; Alc-beta; cadherin-related family member 14; calsyntenin 3; Calsyntenin3; Calsyntenin-3; CDHR14; CLSTN3; CS3; CSTN3; KIAA0726alcbeta; MGC131797; MGC138488
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