Human Calsyntenin-3 Antibody

AF5244 has been discontinued. View all Calsyntenin-3 products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Calsyntenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Calsyntenin-1 and rhCalsyntenin-2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-3
    Lys21-Thr849
    Accession # Q9BQT9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Calsyntenin‑3
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunohistochemistry
Calsyntenin‑3 in Human Brain. Calsyntenin‑3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Calsyntenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5244) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calsyntenin-3

Calsyntenin-3 (CST-3; also CDH-11 and Alcadein-beta ) is a 160 kDa member of the Alcadein family of molecules. It is expressed on the membranes of GABAergic neurons and in the granules of anterior pituitary secretory cells. It is a calcium-binding protein that is proposed to regulate post-synaptic signaling, plus participate in the formation of a membrane-associated APP-X11L-CST-3 complex that blocks APP cleavage. Mature human CST-3 is a 937 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 29-246) in the extracellular region, and an 87 aa cytoplasmic tail. Cleavage presumably occurs, generating a soluble 120 kDa ECD. One splice event shows a 34 aa substitution for the N-terminal 21 amino acids. Over aa 21-849, human CST-3 shares 97% aa identity with mouse CST-3.

  • Entrez Gene IDs:
    9746 (Human); 232370 (Mouse); 171393 (Rat)
  • Alternate Names:
    alcadein beta; alcadein-beta; alcbeta; Alc-beta; cadherin-related family member 14; calsyntenin 3; Calsyntenin3; Calsyntenin-3; CDHR14; CLSTN3; CS3; CSTN3; KIAA0726alcbeta; MGC131797; MGC138488
Related Research Areas

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