Human Carbohydrate Sulfotransferase 4/CHST4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot.
1 Image
Product Details
Supplemental Products

Human Carbohydrate Sulfotransferase 4/CHST4 Antibody Summary

Species Reactivity
Detects human Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4
Accession # Q8NCG5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (Catalog # 5357-ST), see our available Western blot detection antibodies
Measured by its ability to neutralize Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (1.33 µg/mL, Catalog # 5357-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 42 μM) to beta ‑D‑GlcNAc‑(1→6)-alpha -D-Man→OMe (833 μM). The Neutralization Dose (ND50) is typically 10.7 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Carbohydrate Sulfotransferase 4/CHST4 antibody by Western Blot. View Larger

Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5357) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 4/CHST4

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycan (5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.

  1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278:40282.
  2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
  5. Uchimura, K. et al. (2002) J. Biol. Chem. 277:3979.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J. I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39:1613.
  8. Wu, Z.L. et al. (2002) Faseb J. 16:539.
Entrez Gene IDs
10164 (Human); 26887 (Mouse); 307838 (Rat)
Alternate Names
carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; Carbohydrate Sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC; EC; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Carbohydrate Sulfotransferase 4/CHST4 Antibody

There are currently no reviews for this product. Be the first to review Human Carbohydrate Sulfotransferase 4/CHST4 Antibody and earn rewards!

Have you used Human Carbohydrate Sulfotransferase 4/CHST4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review