Detects human Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed.
Polyclonal Sheep IgG
Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4 Leu22-Gln286 Accession # Q8NCG5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Measured by its ability to neutralize Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (1.33 µg/mL, Catalog # 5357-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 42 μM) to beta -D-GlcNAc-(1→6)-alpha -D-Man→OMe (833 μM). The Neutralization Dose (ND50) is typically 10.7 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot.
Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5357) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbohydrate Sulfotransferase 4/CHST4
The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L-selectin ligands. It has a catalytic preference for core 2-branched mucin-type O-glycans, but also has activity toward core 3 type of O-glycan. Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.
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