Human CC Chemokine Receptor D6 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CC Chemokine Receptor D6 in transfectants. Does not stain the parent cell line.
  • Source
    Monoclonal Rat IgG2A Clone # 196124
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL-1 rat basophilic leukemia cell line transfected with human CC Chemokine Receptor D6
    Met1-Ala384
    Accession # O00590
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CC Chemokine Receptor D6 in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CC Chemokine Receptor D6 was stained with Rat Anti-Human CC Chemokine Receptor D6 Monoclonal Antibody (Catalog # MAB1364, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CC Chemokine Receptor D6

D6 is a seven transmembrane receptor that appears to bind to most beta chemokines. D6 expression has been reported on endothelial cells lining afferent lymphatics, placenta and a number of primitive erythromyeloid cell lines.

  • Long Name:
    Chemokine Binding Protein 2
  • Entrez Gene IDs:
    1238 (Human); 59289 (Mouse); 140473 (Rat)
  • Alternate Names:
    ACKR2; C-C chemokine receptor D6; CCBP2; CCR10CCR9D6CC-chemokine-binding receptor JAB61; chemokine (C-C) receptor 9; chemokine binding protein 2; Chemokine receptor CCR-10; Chemokine receptor CCR-9; chemokine receptor D6; chemokine-binding protein 2; Chemokine-binding protein D6; CMKBR9; CMKBR9chemokine (C-C motif) receptor 9; D6; hD6; MGC126678; MGC138250
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding.
    Authors: Hewit, Kay D, Fraser, Alasdair, Nibbs, Robert J, Graham, Gerard J
    J Biol Chem, 2014;289(18):12330-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence.
    Authors: Savino B, Borroni EM, Torres NM, Proost P, Struyf S, Mortier A, Mantovani A, Locati M, Bonecchi R
    J. Biol. Chem., 2009;284(38):26207-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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