Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 196124
View all CC Chemokine Receptor D6 Primary Antibodies »

Product Specifications

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with human CC Chemokine Receptor D6
Met1-Ala384
Accession # O00590

Specificity

Detects human CC Chemokine Receptor D6 in transfectants. Does not stain the parent cell line.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human CC Chemokine Receptor D6 Antibody

Detection of CC Chemokine Receptor D6 antibody in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 antibody by Flow Cytometry.

Detection of CC Chemokine Receptor D6 in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 by Flow Cytometry.

NS0 mouse myeloma cell line transfected with human CC Chemokine Receptor D6 was stained with Rat Anti-Human CC Chemokine Receptor D6 Monoclonal Antibody (Catalog # MAB1364, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CC Chemokine Receptor D6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: NS0 mouse myeloma cell line transfected with human CC Chemokine Receptor D6

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CC Chemokine Receptor D6

D6 is a seven transmembrane receptor that appears to bind to most beta chemokines. D6 expression has been reported on endothelial cells lining afferent lymphatics, placenta and a number of primitive erythromyeloid cell lines.

Long Name

Chemokine Binding Protein 2

Alternate Names

ACKR2, CCBP2, CMKBR9

Entrez Gene IDs

1238 (Human); 59289 (Mouse); 140473 (Rat)

Gene Symbol

ACKR2

UniProt

O00590

Additional CC Chemokine Receptor D6 Products

Product Documents for Human CC Chemokine Receptor D6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CC Chemokine Receptor D6 Antibody

For research use only

Related Research Areas

Endothelial Cell Markers

Citations for Human CC Chemokine Receptor D6 Antibody

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