Human CC Chemokine Receptor D6 Antibody

Catalog # Availability Size / Price Qty
MAB1364
MAB1364-SP
Detection of CC Chemokine Receptor D6 in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 by Flow Cytometry.
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Product Details
Citations (4)
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Human CC Chemokine Receptor D6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CC Chemokine Receptor D6 in transfectants. Does not stain the parent cell line.
Source
Monoclonal Rat IgG2A Clone # 196124
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with human CC Chemokine Receptor D6
Met1-Ala384
Accession # O00590
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CC Chemokine Receptor D6 antibody in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 antibody by Flow Cytometry. View Larger

Detection of CC Chemokine Receptor D6 in NS0 Mouse Cell Line Transfected with Human CC Chemokine Receptor D6 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human CC Chemokine Receptor D6 was stained with Rat Anti-Human CC Chemokine Receptor D6 Monoclonal Antibody (Catalog # MAB1364, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CC Chemokine Receptor D6

D6 is a seven transmembrane receptor that appears to bind to most beta chemokines. D6 expression has been reported on endothelial cells lining afferent lymphatics, placenta and a number of primitive erythromyeloid cell lines.

Long Name
Chemokine Binding Protein 2
Entrez Gene IDs
1238 (Human); 59289 (Mouse); 140473 (Rat)
Alternate Names
ACKR2; C-C chemokine receptor D6; CCBP2; CCR10CCR9D6CC-chemokine-binding receptor JAB61; chemokine (C-C) receptor 9; chemokine binding protein 2; Chemokine receptor CCR-10; Chemokine receptor CCR-9; chemokine receptor D6; chemokine-binding protein 2; Chemokine-binding protein D6; CMKBR9; CMKBR9chemokine (C-C motif) receptor 9; D6; hD6; MGC126678; MGC138250

Product Datasheets

Citations for Human CC Chemokine Receptor D6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding.
    Authors: Hewit, Kay D, Fraser, Alasdair, Nibbs, Robert J, Graham, Gerard J
    J Biol Chem, 2014;289(18):12330-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence.
    Authors: Savino B, Borroni EM, Torres NM, Proost P, Struyf S, Mortier A, Mantovani A, Locati M, Bonecchi R
    J. Biol. Chem., 2009;284(38):26207-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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