Human CCL11/Eotaxin Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB320U Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB320U-100UG

Save 15% on Select RUO Reagents. See Details.

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL11/Eotaxin Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 14, 17, 19, 20, 21, 25, recombinant mouse CCL2, 3, 4, 5,6, 7, 9/10/MIP-1 gamma, 11, 21

Source

Monoclonal Mouse IgG1 Clone # 43911

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CCL11/Eotaxin
Gly24-Pro97
Accession # P51671

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Alternate Names

C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)