Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 14, 17, 19, 20, 21, 25,
recombinant mouse CCL2, 3, 4, 5,6, 7, 9/10/MIP-1 gamma, 11, 21, or 25.
Monoclonal Mouse IgG1 Clone # 43911
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CCL11/Eotaxin Gly24-Pro97 Accession # P51671
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) under non-reducing conditions only
Human CCL11/Eotaxin Biotinylated Antibody (Catalog # BAF320)
Recombinant Human CCL11/Eotaxin Protein (Catalog # 320-EO)
Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CCL11/Eotaxin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CCL11/Eotaxin in Human Skin. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CCL11/Eotaxin in Human Colon. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody. Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse, and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2, and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1-3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).
Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Choe, H. et al. (1996) Cell 85:1135.
Entrez Gene IDs:
6356 (Human); 20292 (Mouse)
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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