Human CCL11/Eotaxin Quantikine ELISA Kit

R&D Systems | Catalog # DTX00

R&D Systems
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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Citrate Plasma (50 µL)

Sensitivity

5 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
View all CCL11/Eotaxin ELISA Kits »

Product Summary for Human CCL11/Eotaxin Quantikine ELISA Kit

The Quantikine Human Eotaxin Immunoassay is a 3.5 hour solid phase ELISA designed to measure human Eotaxin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Eotaxin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human Eotaxin accurately. Results obtained measuring natural human eotaxin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Eotaxin

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 66.8 184 390 67.5 193 396
Standard Deviation 2.0 8.4 23.5 7.8 18.2 40.2
CV% 3.0 4.6 6.0 11.6 9.4 10.2

Citrate Plasma, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 64.5 109 377 63.4 190 400
Standard Deviation 2.5 5.8 12.8 7.3 19.6 33.5
CV% 3.9 5.3 3.4 11.5 10.3 8.4

Recovery for Human CCL11/Eotaxin Quantikine ELISA Kit

The recovery of Eotaxin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 100 86-111
Citrate Plasma (n=5) 99 81-117
EDTA Plasma (n=5) 99 82-115
Serum (n=5) 95 83-109

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of Eotaxin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CCL11/Eotaxin ELISA Linearity

Scientific Data Images for Human CCL11/Eotaxin Quantikine ELISA Kit

Human CCL11/Eotaxin ELISA Cell Culture Supernate Standard Curve

Human CCL11/Eotaxin ELISA Cell Culture Supernate Standard Curve

Human CCL11/Eotaxin ELISA Serum/Plasma Standard Curve

Human CCL11/Eotaxin ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL11/Eotaxin

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

Alternate Names

Eotaxin

Entrez Gene IDs

6356 (Human); 20292 (Mouse)

Gene Symbol

CCL11

Additional CCL11/Eotaxin Products

Product Documents for Human CCL11/Eotaxin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL11/Eotaxin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL11/Eotaxin Quantikine ELISA Kit

Customer Reviews for Human CCL11/Eotaxin Quantikine ELISA Kit (3)

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  • Human CCL11/Eotaxin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 04/20/2022
    Tested human plasma &amp; urine at 4-fold dilution. All urine samples (control &amp; disease) were BLQ. All healthy control plasma was BLQ. Plasma from patient samples was in range.
    Human CCL11/Eotaxin Quantikine ELISA Kit DTX00
  • Human CCL11/Eotaxin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 08/06/2018
  • Human CCL11/Eotaxin Quantikine ELISA Kit
    Name: Alisha Freeman
    Sample Tested: A375 human melanoma cell line
    Verified Customer | Posted 04/19/2018

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Protocols

View specific protocols for Human CCL11/Eotaxin Quantikine ELISA Kit (DTX00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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