Human CCL11/Eotaxin Quantikine ELISA Kit

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Human CCL11/Eotaxin ELISA Cell Culture Supernate Standard Curve
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Product Details
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Citations (34)
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Human CCL11/Eotaxin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)
Sensitivity
5 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human Eotaxin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Eotaxin Immunoassay is a 3.5 hour solid phase ELISA designed to measure human Eotaxin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Eotaxin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human Eotaxin accurately. Results obtained measuring natural human eotaxin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 66.8 184 390 67.5 193 396
Standard Deviation 2 8.4 23.5 7.8 18.2 40.2
CV% 3 4.6 6 11.6 9.4 10.2

Serum, EDTA Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 64.5 109 377 63.4 190 400
Standard Deviation 2.5 5.8 12.8 7.3 19.6 33.5
CV% 3.9 5.3 3.4 11.5 10.3 8.4

Recovery

The recovery of Eotaxin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 100 86-111
Citrate Plasma (n=5) 99 81-117
EDTA Plasma (n=5) 99 82-115
Serum (n=5) 95 83-109

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of Eotaxin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL11/Eotaxin ELISA Linearity

Data Examples

Human CCL11/Eotaxin ELISA Cell Culture Supernate Standard Curve

Human CCL11/Eotaxin ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL11/Eotaxin

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

Entrez Gene IDs:
6356 (Human); 20292 (Mouse)
Alternate Names:
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CCL11/Eotaxin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Reduced ccl11/eotaxin mediates the beneficial effects of environmental stimulation on the aged hippocampus
    Authors: G Scabia, G Testa, M Scali, S Del Turco, G Desiato, N Berardi, A Sale, M Matteoli, L Maffei, M Maffei, M Mainardi, “Train the
    Brain, Behavior, and Immunity, 2021;98(0):234-244.
    Species: Human
    Sample Types: Plasma
  2. Systemic and mucosal immune profiling in asymptomatic and symptomatic SARS-CoV-2-infected individuals reveal unlinked immune signatures
    Authors: S Ravichandr, G Grubbs, J Tang, Y Lee, C Huang, H Golding, S Khurana
    Science Advances, 2021;7(42):eabi6533.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  3. miR-124-3p relieves allergic rhinitis by inhibiting dipeptidyl peptidase-4
    Authors: S Zhang, D Dong, Y Zhang, J Wang, L Liu, Y Zhao
    International immunopharmacology, 2021;101(0):108279.
    Species: Human
    Sample Types: Cell Culture Superantes
  4. miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses
    Authors: F Zhou, P Liu, H Lv, Z Gao, W Chang, Y Xu
    Mol Med Rep, 2021;23(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
    Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
    Allergy Asthma Clin Immunol, 2019;15(0):58.
    Species: Human
    Sample Types: Serum
  6. MicroRNA-874 inhibits TNF-?-induced remodeling in human fetal airway smooth muscle cells by targeting STAT3
    Authors: M Sun, Y Huang, F Li, H Li, B Zhang, L Jin
    Respir Physiol Neurobiol, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Association between plasma CCL11 (eotaxin-1) and cognitive status in older adults: Differences between rural and urban dwellers
    Authors: L Butcher, K Pérès, P André, RH Morris, S Walter, JF Dartigues, L Rodriguez-, C Feart, JD Erusalimsk,
    Exp. Gerontol., 2018;113(0):173-179.
    Species: Human
    Sample Types: Plasma
  8. Profiling of cytokines, chemokines and other soluble proteins as a potential biomarker in colorectal cancer and polyps
    Authors: NA Johdi, L Mazlan, I Sagap, R Jamal
    Cytokine, 2017;99(0):35-42.
    Species: Human
    Sample Types: Serum
  9. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2014;164(2):286-95.
    Species: Human
    Sample Types: Plasma
  10. Increased Eotaxin and MCP-1 Levels in Serum from Individuals with Periodontitis and in Human Gingival Fibroblasts Exposed to Pro-Inflammatory Cytokines.
    Authors: Bostrom E, Kindstedt E, Sulniute R, Palmqvist P, Majster M, Holm C, Zwicker S, Clark R, Onell S, Johansson I, Lerner U, Lundberg P
    PLoS ONE, 2013;10(8):e0134608.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Anti-inflammatory effects of methoxyphenolic compounds on human airway cells.
    Authors: Houser KR, Johnson DK, Ishmael FT
    J Inflamm (Lond), 2012;9(0):6.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
    Authors: Provost V, Langlois A, Chouinard F, Rola-Pleszczynski M, Chakir J, Flamand N, Laviolette M
    PLoS ONE, 2012;7(8):e43544.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
    Authors: Miyagaki T, Sugaya M, Fujita H
    J. Invest. Dermatol., 2010;130(9):2304-11.
    Species: Human
    Sample Types: Serum
  14. Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells.
    Authors: Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB
    Allergy, 2010;65(5):581-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma.
    Authors: Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K
    J. Allergy Clin. Immunol., 2010;126(5):962.
    Species: Human
    Sample Types: Tissue Homogenates
  16. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: BALF
  17. Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis.
    Authors: Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N
    Allergy, 2008;63(10):1359-67.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  18. Effect of IL-13 receptor alpha2 levels on the biological activity of IL-13 variant R110Q.
    Authors: Andrews AL, Bucchieri F, Arima K, Izuhara K, Holgate ST, Davies DE, Holloway JW
    J. Allergy Clin. Immunol., 2007;120(1):91-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
    Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
    J. Allergy Clin. Immunol., 2007;121(2):348-54.
    Species: Human
    Sample Types: Serum
  20. Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
    Authors: Emad A, Emad Y
    J. Clin. Immunol., 2007;27(6):605-12.
    Species: Human
    Sample Types: BALF
  21. Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle.
    Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L199-206.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  23. Cerebrospinal fluid eotaxin and eotaxin-2 levels in human eosinophilic meningitis associated with angiostrongyliasis.
    Authors: Intapan PM, Niwattayakul K, Sawanyawisuth K, Chotmongkol V, Maleewong W
    Cytokine, 2007;39(2):138-41.
    Species: Human
    Sample Types: CSF
  24. Circulating thymus- and activation-regulated chemokine/CCL17 is a useful biomarker for discriminating acute eosinophilic pneumonia from other causes of acute lung injury.
    Authors: Miyazaki E, Nureki S, Ono E, Ando M, Matsuno O, Fukami T, Ueno T, Kumamoto T
    Chest, 2007;131(6):1726-34.
    Species: Human
    Sample Types: BALF
  25. IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
    Authors: Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
    J. Allergy Clin. Immunol., 2006;118(4):858-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Regulatory mechanisms of galectin-9 and eotaxin-3 synthesis in epidermal keratinocytes: possible involvement of galectin-9 in dermal eosinophilia of Th1-polarized skin inflammation.
    Authors: Igawa K, Satoh T, Hirashima M, Yokozeki H
    Allergy, 2006;61(12):1385-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Markers of eosinophilic inflammation and risk prediction in patients with coronary artery disease.
    Authors: Falcone C, Minoretti P, D&amp;amp;apos;Angelo A, Buzzi MP, Coen E, Emanuele E, Aldeghi A, Olivieri V, Geroldi D
    Eur. J. Clin. Invest., 2006;36(4):211-7.
    Species: Human
    Sample Types: Plasma
  28. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
    Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
    J. Immunol., 2006;176(6):3788-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
    Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
    Eur. Respir. J., 2005;25(4):640-6.
    Species: Human
    Sample Types: Sputum
  30. Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response.
    Authors: Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I
    J. Allergy Clin. Immunol., 2005;116(4):805-11.
    Species: Human
    Sample Types: Tissue Secretion
  31. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
    Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
    Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, 107334, Hallden G, Hylander B, Lundahl J
    Clin. Exp. Immunol., 2003;131(1):75-81.
    Species: Human
    Sample Types: Peritoneal Fluid
  33. CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
    Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
    Blood, 2002;100(7):2321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. CCL11 is increased in the CNS in chronic traumatic encephalopathy but not in Alzheimer's disease.
    Authors: Cherry J, Stein T, Tripodis Y, Alvarez V, Huber B, Au R, Kiernan P, Daneshvar D, Mez J, Solomon T, Alosco M, McKee A
    PLoS ONE, 0;12(9):e0185541.
    Species: Human
    Sample Types: CSF

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Reviews for Human CCL11/Eotaxin Quantikine ELISA Kit

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Human CCL11/Eotaxin Quantikine ELISA Kit
By Anonymous on 04/20/2022
Sample Tested: Plasma,Urine

Tested human plasma & urine at 4-fold dilution. All urine samples (control & disease) were BLQ. All healthy control plasma was BLQ. Plasma from patient samples was in range.


Human CCL11/Eotaxin Quantikine ELISA Kit
By Anonymous on 08/06/2018
Sample Tested: Serum

Human CCL11/Eotaxin Quantikine ELISA Kit
By Alisha Freeman on 04/19/2018
Sample Tested: A375 human melanoma cell line