Human CCL22/MDC DuoSet ELISA

Catalog # Availability Size / Price Qty
DY336
Ancillary Products Available
Human CCL22 / MDC ELISA Standard Curve
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Product Details
Procedure
Citations (22)
FAQs
Supplemental Products
Reviews (1)

Human CCL22/MDC DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL22/MDC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Human CCL22 / MDC ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL22/MDC

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.

Entrez Gene IDs:
6367 (Human); 20299 (Mouse)
Alternate Names:
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CCL22/MDC DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability
    Authors: H Janbazacya, J van Bergen, S Varasteh, J Garssen, G Folkerts, S Braber
    Cytokine, 2022;154(0):155878.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Skullcapflavone II Suppresses TNF-alpha/IFN-gamma-Induced TARC, MDC, and CTSS Production in HaCaT Cells
    Authors: H Lee, DH Lee, JH Oh, JH Chung
    International Journal of Molecular Sciences, 2021;22(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. IL-25 Induced ROS-Mediated M2 Macrophage Polarization via AMPK-Associated Mitophagy
    Authors: ML Tsai, YG Tsai, YC Lin, YL Hsu, YT Chen, MK Tsai, WT Liao, YC Lin, CH Hung
    International Journal of Molecular Sciences, 2021;23(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions
    Authors: ZS Wong, SL Sokol-Borr, P Olias, JP Dubey, JP Boyle
    PLoS Pathog., 2020;16(6):e1008528.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Human Placental Syncytiotrophoblasts Restrict Toxoplasma gondii Attachment and Replication and Respond to Infection by Producing Immunomodulatory Chemokines
    Authors: SE Ander, EN Rudzki, N Arora, Y Sadovsky, CB Coyne, JP Boyle
    MBio, 2018;9(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Rhododendron album Blume extract inhibits TNF-?/IFN-?-induced chemokine production via blockade of NF-?B and JAK/STAT activation in human epidermal keratinocytes
    Authors: JW Park, HS Lee, Y Lim, JH Paik, OK Kwon, JH Kim, I Paryanto, P Yunianto, S Choi, SR Oh, KS Ahn
    Int. J. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Biomarkers and immunoprofiles associated with fetal abnormalities of ZIKV-positive pregnancies
    Authors: SS Foo, W Chen, Y Chan, WS Lee, SA Lee, G Cheng, K Nielsen-Sa, P Brasil, JU Jung
    JCI Insight, 2018;3(21):.
    Species: Human
    Sample Types: Serum
  8. CCL22-specific T Cells: Modulating the immunosuppressive tumor microenvironment
    Authors: Evelina Martinenai
    Oncoimmunology, 2016;5(11):e1238541.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Amyloid precursor protein mediated changes in intestinal epithelial phenotype in vitro.
    Authors: Puig K, Manocha G, Combs C
    PLoS ONE, 2016;10(3):e0119534.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice
    PLoS ONE, 2016;11(12):e0167687.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015;10(2):e0116560.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015;10(1):e0116775.
    Species: Human
    Sample Types: BALF
  13. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014;5(0):4092.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. IRF5 promotes inflammatory macrophage polarization and T(H)1-T(H)17 responses.
    Authors: Krausgruber T, Blazek K, Smallie T
    Nat. Immunol., 2011;12(3):231-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae.
    Authors: Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P
    J. Immunol., 2011;186(7):3966-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Divergent effects of hypoxia on dendritic cell functions.
    Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
    Blood, 2008;112(9):3723-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
    Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
    J. Allergy Clin. Immunol., 2006;118(2):354-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Ligation of the FcR gamma chain-associated human osteoclast-associated receptor enhances the proinflammatory responses of human monocytes and neutrophils.
    Authors: Merck E, Gaillard C, Scuiller M, Scapini P, Cassatella MA, Trinchieri G, Bates EE
    J. Immunol., 2006;176(5):3149-56.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
    Authors: Walker J, Tough DF
    Eur. J. Immunol., 2006;36(7):1827-36.
    Species: Human
    Sample Types: Whole Cells
  22. CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells.
    Authors: Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM
    J. Immunol., 2005;174(3):1723-8.
    Species: Human
    Sample Types: Serum

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Human CCL22/MDC DuoSet ELISA
By Teresa Williams on 12/11/2020
Sample Tested: Cell culture supernatant