Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DCC240B
Control Products Available
Human CCL24/Eotaxin-2/MPIF-2 ELISA Cell Culture Supernate Standard Curve
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Product Details
Citations (15)
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Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
14.3 pg/mL
Assay Range
78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Eotaxin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Eotaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Eotaxin-2 levels in cell culture supernates, serum, and plasma. It contains E.coli-expressed recombinant human Eotaxin-2 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-2 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 453 1239 2487 523 1379 2528
Standard Deviation 43 100 188 51.4 74.6 141
CV% 9.6 8 7.6 9.8 5.4 5.6

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 646 1856 3693 750 1971 3506
Standard Deviation 46 121 201 63.2 112 284
CV% 7.1 6.5 5.4 8.4 5.7 8.1

Recovery

The recovery of Eotaxin-2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 78 72-84
EDTA Plasma (n=4) 104 82-117
Heparin Plasma (n=4) 99 80-115
Serum (n=4) 103 84-116

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of Eotaxin-2 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL24/Eotaxin-2/MPIF-2 ELISA Linearity

Scientific Data

Human CCL24/Eotaxin-2/MPIF-2 ELISA Cell Culture Supernate Standard Curve

Human CCL24/Eotaxin-2/MPIF-2 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor 2 (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. Mouse Eotaxin-2 precursor protein shares approximately 58% amino acid sequence identity with human Eotaxin-2.

Entrez Gene IDs:
6369 (Human); 56221 (Mouse)
Alternate Names:
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Downregulation of Salt-Inducible Kinase 3 Enhances CCL24 Activation in the Placental Environment with Preeclampsia
    Authors: Tsai, HF;Tseng, CF;Liang, YL;Wu, PY;Huang, LY;Lin, YH;Lin, LH;Lin, CN;Hsu, KF;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates, Cord Blood Serum
  2. One-Hour Esophageal String Test: A Nonendoscopic Minimally Invasive Test That Accurately Detects Disease Activity in Eosinophilic Esophagitis
    Authors: SJ Ackerman, AF Kagalwalla, I Hirano, N Gonsalves, PM Katcher, S Gupta, JB Wechsler, M Grozdanovi, Z Pan, JC Masterson, J Du, RJ Fantus, P Alumkal, JJ Lee, S Ochkur, F Ahmed, K Capocelli, H Melin-Alda, K Biette, A Dubner, K Amsden, K Keeley, M Sulkowski, A Zalewski, D Atkins, GT Furuta
    Am. J. Gastroenterol., 2019-10-01;114(10):1614-1625.
    Species: Human
    Sample Types: Tissue Homogenates
  3. Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
    Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
    Allergy Asthma Clin Immunol, 2019-09-18;15(0):58.
    Species: Human
    Sample Types: Serum
  4. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2013-10-28;164(2):286-95.
    Species: Human
    Sample Types: Plasma
  5. Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
    Authors: Provost V, Langlois A, Chouinard F, Rola-Pleszczynski M, Chakir J, Flamand N, Laviolette M
    PLoS ONE, 2012-08-31;7(8):e43544.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid.
    Authors: Kagami S, Kai H, Kakinuma T
    J. Invest. Dermatol., 2011-09-01;132(1):249-51.
    Species: Human
    Sample Types: Serum
  7. Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells.
    Authors: Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS
    Cytokine, 2008-11-26;44(3):342-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
    Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
    J. Allergy Clin. Immunol., 2007-12-20;121(2):348-54.
    Species: Human
    Sample Types: Serum
  9. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  10. Cerebrospinal fluid eotaxin and eotaxin-2 levels in human eosinophilic meningitis associated with angiostrongyliasis.
    Authors: Intapan PM, Niwattayakul K, Sawanyawisuth K, Chotmongkol V, Maleewong W
    Cytokine, 2007-08-20;39(2):138-41.
    Species: Human
    Sample Types: CSF
  11. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
    Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
    J. Immunol., 2007-06-15;178(12):7879-89.
    Species: Human
    Sample Types: BALF
  12. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2007-03-09;36(5):237-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
    Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
    J. Immunol., 2006-05-15;176(10):5760-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Markers of eosinophilic inflammation and risk prediction in patients with coronary artery disease.
    Authors: Falcone C, Minoretti P, D&amp;apos;Angelo A, Buzzi MP, Coen E, Emanuele E, Aldeghi A, Olivieri V, Geroldi D
    Eur. J. Clin. Invest., 2006-04-01;36(4):211-7.
    Species: Human
    Sample Types: Plasma
  15. Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions.
    Authors: Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW
    Chest, 2005-06-01;127(6):2094-100.
    Species: Human
    Sample Types: Pleural Effusion

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Reviews for Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit

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Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
By Anonymous on 03/29/2021
Sample Tested: Serum and Plasma

we used this kit to quantify CCL24 in human sera, the kit works well and minimal dilution is required and it produces a very good standard curve.