Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit

(12 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    14.3 pg/mL
  • Assay Range
    78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Eotaxin-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Eotaxin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Eotaxin-2 levels in cell culture supernates, serum, and plasma. It contains E.coli-expressed recombinant human Eotaxin-2 and antibodies raised against the recombinant factor. Results obtained for naturally occurring human Eotaxin-2 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Eotaxin-2.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4310018851.474.6141

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4612120163.2112284


The recovery of Eotaxin-2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 78 72-84
EDTA Plasma (n=4) 104 82-117
Heparin Plasma (n=4) 99 80-115
Serum (n=4) 103 84-116
To assess the linearity of the assay, samples containing or spiked with high concentrations of Eotaxin-2 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL24/Eotaxin-2/MPIF-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor 2 (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. Mouse Eotaxin-2 precursor protein shares approximately 58% amino acid sequence identity with human Eotaxin-2.

    • Entrez Gene IDs
      6369 (Human); 56221 (Mouse);
    • Alternate Names
      chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
      Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
      Br J Haematol, 2014;164(2):286-95.
      Species: Human
      Sample Type: Plasma
    2. High Levels of CCL26 in Blister Fluid and Sera of Patients with Bullous Pemphigoid.
      Authors: Kagami S, Kai H, Kakinuma T
      J. Invest. Dermatol., 2012;132(1):249-51.
      Species: Human
      Sample Type: Serum
    3. Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
      Authors: Provost V, Langlois A, Chouinard F, Rola-Pleszczynski M, Chakir J, Flamand N, Laviolette M
      PLoS ONE, 2012;7(8):e43544.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells.
      Authors: Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS
      Cytokine, 2008;44(3):342-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Systemic responses after bronchial aspirin challenge in sensitive patients with asthma.
      Authors: Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML
      J. Allergy Clin. Immunol., 2007;121(2):348-54.
      Species: Human
      Sample Type: Serum
    6. Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
      Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O&amp;amp;apos;Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
      J. Immunol., 2007;178(12):7879-89.
      Species: Human
      Sample Type: BALF
    7. Cerebrospinal fluid eotaxin and eotaxin-2 levels in human eosinophilic meningitis associated with angiostrongyliasis.
      Authors: Intapan PM, Niwattayakul K, Sawanyawisuth K, Chotmongkol V, Maleewong W
      Cytokine, 2007;39(2):138-41.
      Species: Human
      Sample Type: CSF
    8. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
      Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
      J. Allergy Clin. Immunol., 2007;120(5):1118-25.
      Species: Human
      Sample Type: BALF
    9. Markers of eosinophilic inflammation and risk prediction in patients with coronary artery disease.
      Authors: Falcone C, Minoretti P, D&amp;amp;apos;Angelo A, Buzzi MP, Coen E, Emanuele E, Aldeghi A, Olivieri V, Geroldi D
      Eur. J. Clin. Invest., 2006;36(4):211-7.
      Species: Human
      Sample Type: Plasma
    10. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
      Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
      J. Immunol., 2006;176(10):5760-71.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
      Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
      Cytokine, 2006;36(5):237-44.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions.
      Authors: Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW
      Chest, 2005;127(6):2094-100.
      Species: Human
      Sample Type: Pleural Effusion
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