Human CCL4/MIP-1 beta APC-conjugated Antibody Summary
Accession # P13236
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCL4/MIP-1 beta APC-conjugated Monoclonal Antibody (Catalog # IC271A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL4/MIP-1 beta
CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ), is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.
Citation for Human CCL4/MIP-1 beta APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody-dependent (AD) NK cell activation and ADCC
Authors: I Lisovsky, S Kant, A Tremblay-M, G Isitman, Z Kiani, FP Dupuy, L Gilbert, J Bruneau, NH Shoukry, B Lebouché, NF Bernard
J. Leukoc. Biol., 2019;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
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