Human CCL4/MIP-1 beta MAb (Clone 24006)

Catalog # Availability Size / Price Qty
Chemotaxis Induced by CCL4/MIP‑1 beta  and Neutralization by Human CCL4/MIP‑1 beta  Antibody.
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Product Details
Citations (16)
Supplemental Products

Human CCL4/MIP-1 beta MAb (Clone 24006) Summary

Species Reactivity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human
(rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.
Monoclonal Mouse IgG2B Clone # 24006
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Accession # P13236
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Complete Your Experiment


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME)
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Human CCL4/MIP-1 beta Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL4/MIP‑1 beta Biotinylated Antibody (Catalog # BAF271)

Standard: Recombinant Human CCL4/MIP-1 beta Protein (Catalog # 271-BME)

Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP‑1 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL4/MIP‑1 beta  and Neutralization by Human CCL4/MIP‑1 beta  Antibody. View Larger

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody. Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL4/MIP-1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271). The ND50 is typically 0.3-1.2 µg/mL.

Intracellular Staining by Flow Cytometry Detection of CCL4/MIP-1 beta  antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP-1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL4/MIP-1 beta

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Entrez Gene IDs
6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Citations for Human CCL4/MIP-1 beta MAb (Clone 24006)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. A map of transcriptional heterogeneity and regulatory variation in human microglia
    Authors: AMH Young, N Kumasaka, F Calvert, TR Hammond, A Knights, N Panousis, JS Park, J Schwartzen, J Liu, K Kundu, M Segel, NA Murphy, CE McMurran, H Bulstrode, J Correia, KP Budohoski, A Joannides, MR Guilfoyle, R Trivedi, R Kirollos, R Morris, MR Garnett, I Timofeev, I Jalloh, K Holland, R Mannion, R Mair, C Watts, SJ Price, PJ Kirkpatric, T Santarius, E Mountjoy, M Ghoussaini, N Soranzo, OA Bayraktar, B Stevens, PJ Hutchinson, RJM Franklin, DJ Gaffney
    Nature Genetics, 2021;53(6):861-868.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia
    Authors: A van Hooij, EM Tjon Kon F, M Batista da, R Carvalho B, AC Cunha Mess, AR Gobbo, T Lema, K Bobosha, J Li, X Weng, CG Salgado, JS Spencer, PLAM Corstjens, A Geluk
    Sci Rep, 2018;8(1):17920.
    Species: Human
    Sample Types: Whole Cells
    Applications: Lateral Flow
  3. Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
    Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
    PLoS Pathog., 2018;14(2):e1006888.
  4. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer.
    Authors: Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H
    Clin Cancer Res, 2015;21(10):2268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISpot Development
  5. HIV-1-induced impairment of dendritic cell cross talk with gammadelta T lymphocytes.
    Authors: Cardone M, Ikeda K, Varano B, Gessani S, Conti L
    J Virol, 2015;89(9):4798-808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  7. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008;214(3):328-36.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  9. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
    Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
    Clin. Immunol., 2008;128(1):57-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  10. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  11. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  13. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006;54(7):2096-108.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  14. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
    Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
    J. Virol., 2006;80(1):236-45.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  15. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid
    Applications: Neutralization
  16. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004;75(3):504-14.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry


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