Human CCL4/MIP-1 beta Fluorescein-conjugated Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
  • Source
    Monoclonal Mouse IgG2B Clone # 24006
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
    Ala24-Asn92
    Accession # P13236
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC271F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL4/MIP-1 beta
CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.
  • Entrez Gene IDs:
    6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
  • Alternate Names:
    ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Preservation of Lymphopoietic Potential and Virus Suppressive Capacity by CD8+ T Cells in HIV-2-Infected Controllers
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
    Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
    PLoS Pathog, 2015;11(3):e1004671.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells.
    Authors: Boer M, van Meijgaarden K, Joosten S, Ottenhoff T
    PLoS ONE, 2014;9(4):e94192.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. CD39 is involved in mediating suppression by Mycobacterium bovis BCG-activated human CD8(+) CD39(+) regulatory T cells.
    Authors: Boer M, van Meijgaarden K, Bastid J, Ottenhoff T, Joosten S
    Eur J Immunol, 2013;43(7):1925-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. A molecular basis for the control of preimmune escape variants by HIV-specific CD8+ T cells.
    Authors: Ladell K, Hashimoto M, Iglesias M, Wilmann P, McLaren J, Gras S, Chikata T, Kuse N, Fastenackels S, Gostick E, Bridgeman J, Venturi V, Arkoub Z, Agut H, van Bockel D, Almeida J, Douek D, Meyer L, Venet A, Takiguchi M, Rossjohn J, Price D, Appay V
    Immunity, 2013;38(3):425-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
    Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R
    PLoS Pathog, 2012;8(11):e1003042.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection.
    Authors: Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A
    J. Immunol., 2011;186(9):5151-61.
    Species: Primate - Cercocebus torquatus (Sooty Mangabey)
    Sample Type: Whole Cells
    Application: Flow
  8. CD16- natural killer cells: enrichment in mucosal and secondary lymphoid tissues and altered function during chronic SIV infection.
    Authors: Reeves RK, Gillis J, Wong FE
    Blood, 2010;115(22):4439-46.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  9. Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
    Authors: Guzzo C, Che Mat NF, Gee K
    J. Biol. Chem., 2010;285(32):24404-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008;111(7):3579-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Chemokines and their receptors in whiplash injury: elevated RANTES and CCR-5.
    Authors: Kivioja J, Rinaldi L, Ozenci V, Kouwenhoven M, Kostulas N, Lindgren U, Link H
    J. Clin. Immunol., 2001;21(4):272-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 12 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human CCL4/MIP-1 beta Fluorescein-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information