Human CCR6 Fluorescein-conjugated Antibody

Clone 53103 was used by HLDA to establish CD designation.
(10 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR6. Stains human CCR6 transfectants but not the parental cell line in flow cytometry. Does not cross-react with transfectants expressing human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CXCR1, CXCR2, CXCR3, CXCR4, or STRL33.
  • Source
    Monoclonal Mouse IgG2B Clone # 53103
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR6
    Met1-Met374
    Accession # P51684
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CCR6 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB195F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis.
    Authors: Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S
    J Immunol, 2015;194(11):5139-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CCR2+CCR5+ T Cells Produce Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis.
    Authors: Sato W, Tomita A, Ichikawa D, Lin Y, Kishida H, Miyake S, Ogawa M, Okamoto T, Murata M, Kuroiwa Y, Aranami T, Yamamura T
    J Immunol, 2012;189(10):5057-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor.
    Authors: Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L
    J. Invest. Dermatol., 2010;130(6):1646-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  5. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010;24(4):491-502.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
    Authors: Rohrl J, Yang D, Oppenheim JJ, Hehlgans T
    J. Immunol., 2010;184(12):6688-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009;1(4):4ra10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007;82(4):946-55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007;171(3):767-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. CCR6 colocalizes with CD18 and enhances adhesion to activated endothelial cells in CCR6-transduced Jurkat T cells.
    Authors: Maki W, Morales RE, Carroll VA, Telford WG, Knibbs RN, Stoolman LM, Hwang ST
    J. Immunol., 2002;169(5):2346-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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