Human CCRL2/CRAM-A/B PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.
  • Source
    Monoclonal Mouse IgG2B Clone # 152254
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CRAM-A
    Met1-Val356 (Val180Met)
    Accession # NP_001124382
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry. Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCRL2/CRAM‑A/B PE‑conjugated Monoclonal Antibody (Catalog # FAB23501P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCRL2/CRAM-A/B
CCRL2, also known as CCR11, CRAM-A, CRAM-B, CKRX, and HCR, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. The isoforms CRAM-A and CRAM-B differ at their N-termini by the insertion of an additional 12 amino acids in CRAM-A. CCRL2/CRAM‑A/B is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils, B cells, and T cells (1, 2). CCL19 has been identified as a ligand for CRAM-B (4). Human and mouse CRAM share approximately 47% amino acid sequence identity.
  • References:
    1. Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
    2. Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
    3. Hartmann, T.N. et al. (2008) Immunology 125:252.
    4. Leick, M. et al. (2010) Immunology 129:536.
  • Long Name:
    CC Chemokine Receptor-like 2
  • Entrez Gene IDs:
    9034 (Human)
  • Alternate Names:
    ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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