Human CD11b/Integrin alpha M Alexa Fluor® 700-conjugated Antibody

FAB1699N Datasheet / COA / SDS

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FAB1699N-100UG

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Human CD11b/Integrin alpha M Alexa Fluor® 700-conjugated Antibody in Flow Cytometry

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Human CD11b/Integrin alpha M Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD11b/Integrin alpha M.

Source

Monoclonal Mouse IgG₁ Clone # ICRF44

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human rheumatoid synovial cells and monocytes

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Integrin alpha M/CD11b in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin alpha M/CD11b Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1699N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6