Human CD11b/Integrin alpha M Biotinylated Antibody

Name: Human CD11b/Integrin alpha M Biotinylated Antibody
Brand: R&D Systems
SKU: BAM1699
Price: 389 USD
Availability: InStock

R&D Systems | Catalog # BAM1699

Submit a review

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # ICRF44

View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Human rheumatoid synovial cells and monocytes

Specificity

Detects human CD11b/Integrin alpha M in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human CD11b/Integrin alpha M Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells

Immunohistochemistry

8-25 µg/mL
Sample: Myones, B. et al. (1988) J. Clin Invest. 82: 640. This clone is not recommended for paraffin-embedded sections.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressedon granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement opsonized particles.