Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-reported

Cited:

Immunohistochemistry-Frozen, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ICRF44
View all CD11b/Integrin alpha M Primary Antibodies »

Product Specifications

Immunogen

Human rheumatoid synovial cells and monocytes

Specificity

Detects human CD11b/Integrin alpha M.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD11b/Integrin alpha M Antibody

Detection of CD11b/Integrin alpha M antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CD11b/Integrin alpha M in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1699, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CD11b/Integrin aM antibody in Human PBMCs by Immunocytochemistry (ICC).

CD11b/Integrin alpha M in Human PBMCs.

CD11b/Integrin aM was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB1699) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CD11b/Integrin alpha M Antibody

Application
Recommended Usage

CyTOF-reported

Miner, J.J. et al. (2015) Arthritis and Rheumatol. 67: 1214. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood monocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

Myones, B. et al. (1988) J. Clin Invest. 82: 640.  This clone is not recommended for paraffin-embedded sections.

Reviewed Applications

Read 1 review rated 5 using MAB1699 in the following applications:

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

Additional CD11b/Integrin alpha M Products

Product Documents for Human CD11b/Integrin alpha M Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD11b/Integrin alpha M Antibody

For research use only

Citations for Human CD11b/Integrin alpha M Antibody

Customer Reviews for Human CD11b/Integrin alpha M Antibody (1)

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  • Human CD11b/Integrin alpha M Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/22/2022
    Human CD11b/Integrin alpha M Antibody MAB1699

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Protocols

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