Human CD163 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1607
DY1607-05
Ancillary Products Available
Human CD163 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (24)
FAQs
Supplemental Products
Reviews (5)

Human CD163 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD163. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human CD163 ELISA Standard Curve

Human CD163 DuoSet ELISA Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index (ESSDAI) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome (n = 58). The nine proteins were CXCL13, TNF receptor 2 (TNF-R2), CD48, B-cell activating factor (BAFF), programmed cell death protein 1 ligand 2 (PD-L2), lymphocyte activation gene-3 (LAG-3), beta 2-microglobulin ( beta 2MG), ephrin type-B receptor 2 (EPHB2), and CD163. Pearson’s correlation test was used. The correlation coefficients (r) and P values are shown in the table. P <0.05 was considered significant Image collected and cropped by CiteAb from the following open publication (https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-016-1006-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Entrez Gene IDs:
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names:
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CD163 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV Infection
    Authors: Giron, LB;Liu, Q;Adeniji, OS;Yin, X;Kannan, T;Ding, J;Lu, DY;Langan, S;Zhang, J;Azevedo, JLLC;Hanna, DB;Ofotokun, I;Lazar, J;Fischl, MA;Haberlen, S;Macatangay, B;Adimora, AA;Jamieson, BD;Rinaldo, C;Merenstein, D;Roan, NR;Kutsch, O;Gange, S;Wolinsky, S;Witt, M;Post, WS;Kossenkov, A;Landay, A;Frank, I;Tien, PC;Gross, R;Brown, TT;Abdel-Mohsen, M;
    bioRxiv : the preprint server for biology
    Species:  Human
    Sample Types: Plasma
    Applications: ELISA
  2. The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling
    Authors: M Ackermann, JC Kamp, C Werlein, CL Walsh, H Stark, V Prade, R Surabattul, WL Wagner, C Disney, AJ Bodey, T Illig, DJ Leeming, MA Karsdal, A Tzankov, P Boor, MP Kühnel, FP Länger, SE Verleden, HM Kvasnicka, HH Kreipe, A Haverich, SM Black, A Walch, P Tafforeau, PD Lee, MM Hoeper, T Welte, B Seeliger, S David, D Schuppan, SJ Mentzer, DD Jonigk
    EBioMedicine, 2022-10-04;85(0):104296.
    Species: Human
    Sample Types: Plasma
  3. Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults
    Authors: H Kared, AS Wolf, A Alirezayla, A Ravussin, G Solum, TT Tran, F Lund-Johan, JT Vaage, LS Nissen-Mey, UC Nygaard, O Hungnes, AH Robertson, LM Næss, L Trogstad, P Magnus, LA Munthe, S Mjaaland
    Nature Communications, 2022-07-18;13(1):4165.
    Species: Human
    Sample Types: Plasma
  4. The performance of soluble CD163 as a non-invasive biomarker of liver damage in chronically HCV and HCV/HIV infected subjects
    Authors: V Cairoli, E De Matteo, P Casciato, B Ameigeiras, MV Preciado, P Valva
    PLoS ONE, 2022-07-07;17(7):e0270911.
    Species: Human
    Sample Types: Plasma
  5. Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Authors: RB da Silva P, MA Mendes, VC Soares, J França-Cos, AM Sales, NC Duppré, V de Matos B, TP da Silva, PT Bozza, MT Bozza, EN Sarno, MO Moraes, GM Sperandio, RO Pinheiro
    Scientific Reports, 2022-05-12;12(1):7850.
    Species: Human
    Sample Types: Serum
  6. A 3D Microfluidic ELISA for the Detection of Severe Dengue: Sensitivity Improvement and Vroman Effect Amelioration by EDC-NHS Surface Modification
    Authors: H Maeno, PF Wong, S AbuBakar, M Yang, SS Sam, J Jamil-Abd, A Shunmugara, M Mustafa, RM Said, E Mageswaren, A Azmel, A Mat Jelani
    Micromachines, 2021-11-30;12(12):.
    Species: Human
    Sample Types: Serum
  7. Inflammatory pathways amongst people living with HIV in Malawi differ according to socioeconomic status
    Authors: C Kelly, W Tinago, D Alber, P Hunter, N Luckhurst, J Connolly, F Arrigoni, A Garcia Abn, R Kamn'gona, I Sheha, M Chammudzi, K Jambo, J Mallewa, A Rapala, PWG Mallon, H Mwandumba, N Klein, S Khoo
    PLoS ONE, 2021-08-25;16(8):e0256576.
    Species: Human
    Sample Types: Plasma
  8. Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients
    Authors: HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas
    PLoS ONE, 2020-12-16;15(12):e0243759.
    Species: Human
    Sample Types: Plasma
  9. Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection
    Authors: S Novelli, C Lécuroux, C Goujard, J Reynes, A Villemant, L Blum, A Essat, V Avettand-F, O Launay, JM Molina, C Bourgeois, L Meyer
    EBioMedicine, 2020-11-26;62(0):103129.
    Species: Human
    Sample Types: Plasma
  10. HIV-associated gut dysbiosis is independent of sexual practice and correlates with noncommunicable diseases
    Authors: I Vujkovic-C, O Sortino, E Verheij, J Sklar, FW Wit, NA Kootstra, B Sellers, JM Brenchley, J Ananworani, MSV Loeff, Y Belkaid, P Reiss, I Sereti
    Nat Commun, 2020-05-15;11(1):2448.
    Species: Human
    Sample Types: Plasma
  11. Biofluid quantification of TWEAK/Fn14 axis in combination with a selected biomarker panel improves assessment of prostate cancer aggressiveness
    Authors: X Ruiz-Plaza, E Rodríguez-, M Alves, A Altuna-Coy, J Lozano-Bar, M Portero-Ot, JF García-Fon, S Martínez-G, J Segarra, MR Chacón
    J Transl Med, 2019-09-09;17(1):307.
    Species: Human
    Sample Types: Serum
  12. Soluble CD163 and TWEAK in early pregnancy gestational diabetes and later glucose intolerance
    Authors: J Dereke, J Nilsson, C Nilsson, H Strevens, M Landin-Ols, M Hillman
    PLoS ONE, 2019-05-09;14(5):e0216728.
    Species: Human
    Sample Types: Plasma
  13. Prevalence and Outcome of Secondary Hemophagocytic Lymphohistiocytosis Among SIRS Patients: Results from a Prospective Cohort Study
    Authors: GA Gualdoni, GA Hofmann, P Wohlfarth, HM Winkler, S Winkler, H Haslacher, R Thalhammer, A Makristath, F Ratzinger, H Burgmann
    J Clin Med, 2019-04-19;8(4):.
    Species: Human
    Sample Types: Plasma
  14. Urinary and serum soluble CD25 complements urinary soluble CD163 to detect active renal anti-neutrophil cytoplasmic autoantibody-associated vasculitis: a cohort study
    Authors: GJ Dekkema, WH Abdulahad, T Bijma, SM Moran, L Ryan, MA Little, CA Stegeman, P Heeringa, JF Sanders
    Nephrol. Dial. Transplant., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Serum
  15. Biomarkers and immunoprofiles associated with fetal abnormalities of ZIKV-positive pregnancies
    Authors: SS Foo, W Chen, Y Chan, WS Lee, SA Lee, G Cheng, K Nielsen-Sa, P Brasil, JU Jung
    JCI Insight, 2018-11-02;3(21):.
    Species: Human
    Sample Types: Serum
  16. Epigenetic alterations are associated with monocyte immune dysfunctions in HIV-1 infection
    Authors: MS Espíndola, LS Soares, LJ Galvão-Lim, FA Zambuzi, MC Cacemiro, VS Brauer, CM Marzocchi-, M de Souza G, LR Amaral, OA Martins-Fi, VR Bollela, FG Frantz
    Sci Rep, 2018-04-03;8(1):5505.
    Species: Human
    Sample Types: Plasma
  17. Impact of early cART on HIV blood and semen compartments at the time of primary infection
    Authors: A Chéret, C Durier, A Mélard, M Ploquin, J Heitzmann, C Lécuroux, V Avettand-F, L David, G Pialoux, JM Chennebaul, M Müller-Tru, C Goujard, C Rouzioux, L Meyer
    PLoS ONE, 2017-07-14;12(7):e0180191.
    Species: Human
    Sample Types: Plasma
  18. Monocyte/macrophage and T cell activation markers are not independently associated with MI risk in healthy individuals - results from the HUNT Study
    Authors: T Ueland, LE Laugsand, LJ Vatten, I Janszky, C Platou, AE Michelsen, JK Damås, P Aukrust, BO Åsvold
    Int. J. Cardiol., 2017-06-12;0(0):.
    Species: Human
    Sample Types: Serum
  19. High Cellular Monocyte Activation in People Living With Human Immunodeficiency Virus on Combination Antiretroviral Therapy and Lifestyle-Matched Controls Is Associated With Greater Inflammation in Cerebrospinal Fluid
    Authors: T Booiman, FW Wit, I Maurer, D De Frances, CA Sabin, AM Harskamp, M Prins, P Garagnani, C Pirazzini, C Franceschi, D Fuchs, M Gisslén, A Winston, P Reiss, NA Kootstra
    Open Forum Infect Dis, 2017-05-25;4(3):ofx108.
    Species: Human
    Sample Types: Plasma
  20. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017-05-25;9(0):41.
    Species: Human
    Sample Types: Plasma
  21. Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis
    Cytokine, 2016-08-31;88(0):99-107.
    Species: Human
    Sample Types: Plasma
  22. Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset
    PLoS Pathog, 2016-08-10;12(8):e1005774.
    Species: Human
    Sample Types: Plasma
  23. The Human Endogenous Protection System against Cell-Free Hemoglobin and Heme Is Overwhelmed in Preeclampsia and Provides Potential Biomarkers and Clinical Indicators.
    Authors: Gram M, Anderson U, Johansson M, Edstrom-Hagerwall A, Larsson I, Jalmby M, Hansson S, Akerstrom B
    PLoS ONE, 2015-09-14;10(9):e0138111.
    Species: Human
    Sample Types: Plasma
  24. Serum sCD163 levels are associated with type 2 diabetes mellitus and are influenced by coffee and wine consumption: results of the Di@bet.es study.
    Authors: Rojo-Martinez G, Maymo-Masip E, Rodriguez M, Solano E, Goday A, Soriguer F, Valdes S, Chaves F, Delgado E, Colomo N, Hernandez P, Vendrell J, Chacon M
    PLoS ONE, 2014-06-30;9(6):e101250.
    Species: Human
    Sample Types: Serum

FAQs

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Reviews for Human CD163 DuoSet ELISA

Average Rating: 4.6 (Based on 5 Reviews)

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Human CD163 DuoSet ELISA
By Anonymous on 10/11/2023
Sample Tested: Plasma

Human CD163 DuoSet ELISA
By Anonymous on 07/03/2023
Sample Tested: Serum

Human CD163 DuoSet ELISA
By Anonymous on 11/07/2022
Sample Tested: Serum

Human CD163 DuoSet ELISA
By Anonymous on 09/13/2019
Sample Tested: Serum and Plasma

We have been using this kit for almost 2 years. I would say this an excellent kit for serum samples. It has a user-friendly protocol which allows researchers to perform assay without any interruptions.


Human CD163 DuoSet ELISA
By Jonatan Dereke on 05/19/2016
Sample Tested: EDTA Plasma,Serum

We have run over three complete CD163 DuoSet ELISA kits so far and the performance of the kit is great. Since we run human serum and plasma, we always dilute the samples 1:200 in reagent diluent 1x.