CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
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Human CD163 Quantikine ELISA Kit
R&D Systems | Catalog # DC1630
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.613 ng/mL
Assay Range
1.56-100 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
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Product Summary for Human CD163 Quantikine ELISA Kit
The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human CD163
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human CD163 in this assay. No medical histories were available for the donors used in this
study.
Cell Culture Supernates:
ND=Non detectable
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=36) | 472 | 88-902 | 186 |
| Heparin plasma (n=36) | 521 | 186-996 | 203 |
Cell Culture Supernates:
PBLs human peripheral blood lymphocytes (1 x 106
cells/mL) were cultured in DMEM
supplemented with 5% fetal bovine serum, 5 μM beta -mercaptoethanol, 2 mM L-glutamine,
100 U/mL of penicillin, and 100 μg/mL of streptomycin sulfate. Cells were cultured
unstimulated or stimulated with 10 μg/mL of PHA. Aliquots of the cell culture supernates
were removed and assayed for levels of human CD163.
| Condition | Day 1 (ng/mL) | Day 6 (ng/mL) |
| Unstimulated | ND | 5.0 |
| Stimulated | ND | 4.4 |
Human monocytes were cultured in RPMI supplemented with 10% fetal bovine serum,
2 mM L-glutamine, 100 U/mL of penicillin, 100 μg/mL of streptomycin sulfate, 10 ng/mL of
recombinant human IFN-gamma, 7.0 ng/mL of recombinant human IL-10, and 5.0 μg/mL of
lipopolysaccharide (LPS). An aliquot of the cell culture supernate was removed, assayed for
human CD163, and measured 64.9 ng/mL.
Human monocytes were cultured in RPMI supplemented with 10% fetal bovine serum and
25 ng/mL of recombinant human GM-CSF. In the last 24 hours, they were stimulated with
50 ng/mL of LPS. An aliquot of the cell culture supernate was removed, assayed for
human CD163, and measured 20.3 ng/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 20.0 | 35.1 | 65.6 | 20.0 | 34.9 | 63.6 |
| Standard Deviation | 0.75 | 1.2 | 2.3 | 1.3 | 1.6 | 2.6 |
| CV% | 3.8 | 3.4 | 3.5 | 6.7 | 4.6 | 4.1 |
Recovery for Human CD163 Quantikine ELISA Kit
The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 101-110 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD163 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CD163 Quantikine ELISA Kit
Human CD163 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD163
Alternate Names
CD163, GHI/61, HbSR, M130, RM3/1
Gene Symbol
CD163
Additional CD163 Products
Product Documents for Human CD163 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CD163 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human CD163 Quantikine ELISA Kit
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Sample Tested: SerumVerified Customer | Posted 07/14/2023
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Sample Tested: SerumVerified Customer | Posted 11/02/2022
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Sample Tested: SerumVerified Customer | Posted 11/02/2022
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Sample Tested: Citrate Plasma, Human serum and patient CSFVerified Customer | Posted 01/07/2021
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Sample Tested: SerumVerified Customer | Posted 09/12/2020
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Sample Tested: EDTA PlasmaVerified Customer | Posted 12/15/2016Plasma was isolated from blood drawn in EDTA tubes from HIV+/ART and HIV- healthy controls. sCD163 was measured using the Quantikine elisa kit for human sCD163 (cat# DC1630) following the protocol supplied in the kit. Plasma samples were diluted 1:10 before use in the assay. Figure legends: a) Standard curve for sCD163 from which concentrations in plasma were extrapolated b) Quantitated data from HIV+/ART and HIV- group. Concentrations are expressed in ng/ml.
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Protocols
View specific protocols for Human CD163 Quantikine ELISA Kit (DC1630):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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