Human CD163 Quantikine ELISA Kit

(11 citations)
(1 Review)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.613 ng/mL
  • Assay Range
    1.6 - 100 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant human CD163
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC61, Quantikine Immunoassay Control Set 903 for Human CD163 - Please Inquire
Product Summary
The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean2035.165.62034.963.6
Standard Deviation0.751.22.31.31.62.6
CV%3.83.43.56.74.64.1

Recovery

The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 101-110
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD163 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CD163 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
    • Entrez Gene IDs
      9332 (Human); 93671 (Mouse); 312701 (Rat);
    • Alternate Names
      CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    11 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV
      Authors: SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke
      Pathog Immun, 2017;2(1):138-150.
      Species: Human
      Sample Type: Plasma
    2. Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
      Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
      PLoS ONE, 2017;12(6):e0179400.
      Species: Human
      Sample Type: Plasma
    3. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
      Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
      Open Forum Infect Dis, 2016;3(4):ofw224.
      Species: Human
      Sample Type: Plasma
    4. Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease
      PLoS ONE, 2016;11(11):e0166954.
      Species: Human
      Sample Type: Plasma
    5. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
      Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
      PLoS ONE, 2015;10(12):e0144317.
      Species: Human
      Sample Type: Serum
    6. Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
      Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
      PLoS ONE, 2014;9(12):e115226.
      Species: Human
      Sample Type: Plasma
    7. Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
      Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
      PLoS ONE, 2014;9(8):e105442.
      Species: Human
      Sample Type: Plasma
    8. Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system.
      Authors: Forsvoll J, Kristoffersen E, Oymar K
      Pediatr Rheumatol Online J, 2013;11(1):38.
      Species: Human
      Sample Type: Plasma
    9. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
      Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
      J Immunol, 2013;190(10):5267-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
      Authors: Shimizu M, Yachie A
      Cytokine, 2012;60(1):226-32.
      Species: Human
      Sample Type: Serum
    11. Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
      Authors: Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O
      Arterioscler. Thromb. Vasc. Biol., 2010;30(6):1253-62.
      Species: Human
      Sample Type: Plasma
    Expand to show all 11 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 903 for Human CD163

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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Average Rating: 4 (Based on 1 review)

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     Human CD163 Quantikine ELISA Kit DC1630
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      Anonymous 12/15/2016
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     Human CD163 Quantikine ELISA Kit DC1630
    : Human CD163 Quantikine ELISA Kit [DC1630]

    Summary

    Sample TestedEDTA Plasma

    Other Experimental Details

    Other Experimental DetailsPlasma was isolated from blood drawn in EDTA tubes from HIV+/ART and HIV- healthy controls. sCD163 was measured using the Quantikine elisa kit for human sCD163 (cat# DC1630) following the protocol supplied in the kit. Plasma samples were diluted 1:10 before use in the assay.Figure legends:a) Standard curve for sCD163 from which concentrations in plasma were extrapolatedb) Quantitated data from HIV+/ART and HIV- group.Concentrations are expressed in ng/ml.

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