Human CD164 Antibody Summary
Accession # Q04900
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CD164 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.
Citation for Human CD164 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
PLoS Genet, 2015;11(7):e1005386.
Sample Types: Cell Lysates
Applications: Western Blot
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