Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CD164 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD164 extracellular domain
Asp24-Leu197
Accession # Q04900

Specificity

Detects human CD164 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD164 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CD164 Antibody

Detection of Human CD164 antibody by Western Blot.

Detection of Human CD164 by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2

Applications for Human CD164 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD164

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

Alternate Names

CD164, MGC-24, MUC-24

Entrez Gene IDs

8763 (Human); 53599 (Mouse)

Gene Symbol

CD164

UniProt

Q04900

Additional CD164 Products

Product Documents for Human CD164 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD164 Antibody

For research use only

Citations for Human CD164 Antibody

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