Human CD200 PerCP-conjugated Antibody

FAB27241C has been discontinued. View all CD200 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD200 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD200 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 325516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD200
    Gln31-Gly232
    Accession # P41217.3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD200 in Th17 Differentiated Human PBMCs by Flow Cytometry. Th17 differentiated humanperipheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD200 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB27241C) and Mouse Anti-Human CD3 epsilon Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins
Detection of CD200 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CD200 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB27241C) or (B) Mouse IgG1 PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (3). A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed (4). Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (5‑7). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (8). CD200 and CD200R associate via their respective N-terminal Ig-like domains (9). In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact (6, 7, 10). In T cells, however, CD200 functions as a co‑stimulatory molecule independent of the CD28 pathway (11). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (12, 13). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (14, 15). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (5, 14‑16).

  • References:
    1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
    2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
    3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
    4. Wright, G.J. et al. (2001) Immunology 102:173.
    5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
    6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
    7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
    8. Hoek, R.M. et al. (2000) Science 290:1768.
    9. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
    10. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
    11. Borriello, F. et al. (1997) J. Immunol. 158:4548.
    12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
    13. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
    14. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
    15. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
    16. Langlais, C.L. et al. (2006) J. Virol. 80:3098.
  • Entrez Gene IDs:
    4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)
  • Alternate Names:
    antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2
Related Research Areas

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