Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CD27 Ligand/TNFSF7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7
Gln45-Pro193
Accession # AAA36175

Specificity

Detects human CD27 Ligand/TNFSF7 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CD27 Ligand and recombinant human CD30 Ligand is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD27 Ligand/TNFSF7 Biotinylated Antibody

Detection of Human CD27 Ligand/TNFSF7 antibody by Western Blot.

Detection of Human CD27 Ligand/TNFSF7 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD27 Ligand/TNFSF7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD27 Ligand/TNFSF7 at approximately 33-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human CD27 Ligand/TNFSF7 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD27 Ligand/TNFSF7

Human CD27 Ligand (also CD70) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.

Alternate Names

CD70, TNFSF7

Entrez Gene IDs

970 (Human); 21948 (Mouse); 301132 (Rat)

Gene Symbol

CD70

UniProt

AAA36175

Additional CD27 Ligand/TNFSF7 Products

Product Documents for Human CD27 Ligand/TNFSF7 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD27 Ligand/TNFSF7 Biotinylated Antibody

For research use only

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