Human CD27 Ligand/TNFSF7 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2738
Detection of Human CD27 Ligand/TNFSF7 by Western Blot.
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Human CD27 Ligand/TNFSF7 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD27 Ligand/TNFSF7 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CD27 Ligand and recombinant human CD30 Ligand is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7
Gln45-Pro193
Accession # AAA36175
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD27 Ligand/TNFSF7 antibody by Western Blot. View Larger

Detection of Human CD27 Ligand/TNFSF7 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD27 Ligand/TNFSF7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD27 Ligand/TNFSF7 at approximately 33-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27 Ligand/TNFSF7

Human CD27 Ligand (also CD70) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.

Entrez Gene IDs
970 (Human); 21948 (Mouse); 301132 (Rat)
Alternate Names
CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7

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