Human CD27 Ligand/TNFSF7 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD27 Ligand/TNFSF7 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CD27 Ligand and recombinant human CD30 Ligand is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7
    Gln45-Pro193
    Accession # AAA36175
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD27 Ligand/TNFSF7 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD27 Ligand/TNFSF7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD27 Ligand/TNFSF7 at approximately 33-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD27 Ligand/TNFSF7
Human CD27 Ligand (also CD70) is a 30 kDa type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20 amino acid (aa) cytoplasmic region and a 155 aa extracellular domain that shows multiple beta -strands. It is an inducible trimer that occurs on B cells, T cells, and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions.
  • Entrez Gene IDs:
    970 (Human); 21948 (Mouse)
  • Alternate Names:
    CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Related Research Areas
Isotype Controls
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