Human CD300f/LMIR3 Antibody

Catalog # Availability Size / Price Qty
MAB2774
MAB2774-SP
CD300f/LMIR3 in Human PBMCs.
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Product Details
Citations (2)
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Human CD300f/LMIR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD300f/LMIR3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 4, 5, 6, or recombinant mouse LMIR3 is observed.
Source
Monoclonal Rat IgG2B Clone # 234903
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CD300f/LMIR3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human whole blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CD300f/LMIR3 in Human PBMCs. View Larger

CD300f/LMIR3 in Human PBMCs. CD300f/LMIR3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Rat Anti-Human CD300f/LMIR3 Monoclonal Antibody (Catalog # MAB2774) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300f/LMIR3

CD300f, also known as CD300LF, LMIR3, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50‑60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human CD300f consists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences (2, 3). Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment (3). Within the ECD, human CD300f shares 43% aa sequence identity with mouse and rat CD300f. CD300f is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts (2‑4). Pervanadate treatment or antibody crosslinking of CD300f induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K (2, 3, 5, 6). CD300f ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9 (3‑8). In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma (5). In mouse, a splice variant of CD300f (known as DIgR2, with a 7 aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses (9). CD300f is upregulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS (10).

References
  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Sui, L. et al. (2004) Biochem. Biophys. Res. Commun. 319:920.
  3. Alvarez-Errico, D. et al. (2004) Eur. J. Immunol. 34:3690.
  4. Korver, W. et al. (2009) Leukemia 23:1587.
  5. Izawa, K. et al. (2009) J. Immunol. 183:925.
  6. Alvarez-Errico, D. et al. (2007) J. Immunol. 178:808.
  7. Can, I. et al. (2008) J. Immunol. 180:207.
  8. Izawa, K. et al. (2007) J. Biol. Chem. 282:17997.
  9. Shi, L. et al. (2006) Blood 108:2678.
  10. Xi, H. et al. (2010) J. Exp. Med. 207:7.
Long Name
Leukocyte Mono Ig-like Receptor 3
Entrez Gene IDs
146722 (Human); 246746 (Mouse)
Alternate Names
CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3

Product Datasheets

Citations for Human CD300f/LMIR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sphingomyelin and ceramide are physiological ligands for human LMIR3/CD300f, inhibiting FcepsilonRI-mediated mast cell activation.
    Authors: Izawa K, Isobe M, Matsukawa T, Ito S, Maehara A, Takahashi M, Yamanishi Y, Kaitani A, Oki T, Okumura K, Kitamura T, Kitaura J
    J Allergy Clin Immunol, 2014;133(1):270-3.e1-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: WB
  2. The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model.
    Authors: Asai, Tsuneaki, Morrison, Sherie L
    J Biol Chem, 2013;288(15):10395-405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

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