Human CD300g/Nepmucin Antibody

Catalog # Availability Size / Price Qty
AF5328
AF5328-SP
Detection of Human CD300g/Nepmucin by Western Blot.
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Human CD300g/Nepmucin Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD300g/Nepmucin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD300g/Nepmucin
Leu19-Leu249
Accession # Q6UXG3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human CD300g/Nepmucin by Western Blot. View Larger

Detection of Human CD300g/Nepmucin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD300g/Nepmucin at approximately 75-95 kDa and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300g/Nepmucin

Nepmucin (A mucin not expressed in Peyer's Patches; also CD300g, CLM9,  and TREM4) is a 75-95 kDa, O-glycosylated member of the CD300 family of molecules. It is expressed on capillary endothelium, and appears to serve multiple functions. Via a mucin domain, it binds lymphocyte L-selectin, contributing to cell migration. It also binds immunoglobulins A and M, perhaps mediating transendothelial transport. Mature human nepmucin is a 314 amino acid (aa ) type I transmembrane glycoprotein. It contains one V-type Ig-like domain (aa 19-121), a mucin stalk (aa 137-237), and a cytoplasmic region (aa 269-332). There are at least two isoform variants of nepmucin. Each shows a deletion of aa 128-212, accompanied by either a 23 aa, or 12 aa substitution for aa 296-332. Over aa 19-249, human nepmucin shares 51% aa identity with mouse nepmucin.

Entrez Gene IDs
146894 (Human); 52685 (Mouse); 684984 (Rat)
Alternate Names
CD300 antigen like family member G; CD300 antigen-like family member G; CD300 molecule like family member g; CD300 molecule-like family member g; CD300g antigen; CD300g; CD300LG; CLM9; CLM-9; CLM9Trem4; CMRF35-like molecule 9; Nepmucin; TREM4; TREM-4; Triggering receptor expressed on myeloid cells 4

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