Human CD300g/Nepmucin Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human CD300g/Nepmucin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD300g/Nepmucin at approximately 75-95 kDa and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage

Background: CD300g/Nepmucin

Nepmucin (A mucin not expressed in Peyer's Patches; also CD300g, CLM9,  and TREM4) is a 75-95 kDa, O-glycosylated member of the CD300 family of molecules. It is expressed on capillary endothelium, and appears to serve multiple functions. Via a mucin domain, it binds lymphocyte L-selectin, contributing to cell migration. It also binds immunoglobulins A and M, perhaps mediating transendothelial transport. Mature human nepmucin is a 314 amino acid (aa ) type I transmembrane glycoprotein. It contains one V-type Ig-like domain (aa 19-121), a mucin stalk (aa 137-237), and a cytoplasmic region (aa 269-332). There are at least two isoform variants of nepmucin. Each shows a deletion of aa 128-212, accompanied by either a 23 aa, or 12 aa substitution for aa 296-332. Over aa 19-249, human nepmucin shares 51% aa identity with mouse nepmucin.

Product Datasheets

Related Research Areas

• Endothelial Cell Markers
• Immune Regulatory Molecules on Endothelial Cells
• Special Glycoproteins