|Detection of Human CD302/CLEC13A by Western Blot. Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6367) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of CD302/CLEC13A in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Sheep Anti-Human CD302/CLEC13A Affinity Purified Polyclonal Antibody (Catalog # AF6367, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).|
CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.