Human CD302/CLEC13A Antibody

Catalog # Availability Size / Price Qty
Detection of Human CD302/CLEC13A by Western Blot.
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Product Details
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Human CD302/CLEC13A Antibody Summary

Species Reactivity
Detects human CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD302 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived human CD302/CLEC13A
Accession # Q8IX05
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD302/CLEC13A antibody by Western Blot. View Larger

Detection of Human CD302/CLEC13A by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6367) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD302/CLEC13A antibody in Human Monocytes antibody by Flow Cytometry. View Larger

Detection of CD302/CLEC13A in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Sheep Anti-Human CD302/CLEC13A Affinity Purified Polyclonal Antibody (Catalog # AF6367, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD302/CLEC13A

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein.  It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205.  CD302 contributes aa 23-232 to the fusion protein.  There is one potential alternative start site at Met63.  Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.

Entrez Gene IDs
9936 (Human); 66205 (Mouse); 295629 (Rat)
Alternate Names
BIMLEC; CD302 antigenC-type lectin domain family 13, member A; CD302 molecule; CD302; CLEC13A; CLEC13AMGC22301; C-type lectin domain family 13 member A; DCL1; DCL-1; DCL1C-type lectin BIMLEC; KIAA0022FLJ43091; Type I transmembrane C-type lectin receptor DCL-1

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