Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4, recombinant feline CD4, recombinant cotton rat CD4, and recombinant canine CD4 is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 Lys26-Trp390 Accession # P01730
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and A) Goat Anti-Human CD4 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165N) or B) isotype control antibody (Catalog # IC108N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
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