Human CD4 Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4, recombinant feline CD4, recombinant cotton rat CD4, and recombinant canine CD4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
    Lys26-Trp390
    Accession # P01730
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and A) Goat Anti-Human CD4 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165N) or B) isotype control antibody (Catalog # IC108N). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

  • References:
    1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
  • Entrez Gene IDs:
    920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
  • Alternate Names:
    CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Alexa Fluor® 700-conjugated Control

Ctrl IC108N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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