Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry|
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). ) and A) Goat Anti-Human CD4 Alexa Fluor® 700‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165N) or B) isotype control antibody (Catalog # IC108N). View our protocol for Staining Membrane-associated Proteins.
CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.