Human CD4 Antibody

Catalog # Availability Size / Price Qty
AF-379-NA
AF-379-SP
CD4 in Human T Cells.
3 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)

Human CD4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Lys26-Trp390
Accession # P01730
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD4 (Catalog # 514-CD)
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil tissue
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry CD4 antibody in Human T Cells by Immunocytochemistry (ICC). View Larger

CD4 in Human T Cells. CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry CD4 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD4 antibody in Human Tonsil Tissue by Immunohistochemistry (IHC-P). View Larger

CD4 in Human Tonsil Tissue. CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References
  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names
CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

Citations for Human CD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma
    Authors: R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan
    Journal of personalized medicine, 2022;12(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses
    Authors: K Sangare, S Helmold Ha, M Moore, C Hogge, T Hoang, MA Rahman, DJ Venzon, C LaBranche, D Montefiori, M Robert-Gur, MA Thomas
    Vaccines, 2022;10(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
    Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
    Biology, 2021;10(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia
    Authors: JP Sutherland, M Steain, ME Buckland, M Rodriguez, AL Cunningham, B Slobedman, A Abendroth
    Front Microbiol, 2019;10(0):2117.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
    Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
    J Clin Invest, 2015;125(2):739-51.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells.
    Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S
    J Virol, 2014;88(19):11045-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo.
    Authors: Suzuki, Yasuhiro, Gatanaga, Hiroyuki, Tachikawa, Natsuo, Oka, Shinichi
    PLoS ONE, 2014;9(2):e86479.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
    Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
    J Allergy Clin Immunol, 2013;131(4):1117-29, 1129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
    Authors: Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J
    PLoS Pathog, 2012;8(9):e1002937.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells.
    Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V
    J. Immunol., 2009;183(7):4792-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies.
    Authors: Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE
    Arthritis Rheum., 2007;56(1):372-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress.
    Authors: Bjerke SL, Roller RJ
    Virology, 2006;347(2):261-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
    Authors: Wang B, He J, Liu C, Chang LJ
    Vaccine, 2006;24(17):3477-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human CD4 Antibody

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Human CD4 Antibody
By Anonymous on 04/06/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Colon cancer tissue Species: Human

Human CD4 Antibody
By Anonymous on 12/02/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Tonsil tissue Species: Human and Human tonsil