Human CD58/LFA-3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD58/LFA‑3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 248310
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3
    Accession # P19256
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD58/LFA‑3 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD58/LFA‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB1689A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human cells and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2‑4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cell cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).

  • References:
    1. Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177.
    2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
    3. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939.
    4. Crawford, K. et al. (2003) Blood 102:1745.
    5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
    6. Bullens, M.A. et al. (2001) Int. Immunol. 12:181.
  • Entrez Gene IDs:
    965 (Human)
  • Alternate Names:
    Ag3; CD58 antigen; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; CD58; FLJ23181; FLJ43722; LFA3; LFA-3; LFA3ag3; lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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