Human CD69 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB23591A

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Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.
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Human CD69 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.
Source
Monoclonal Mouse IgG2A Clone # 298614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD69 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either untreated (light orange filled histogram) or treated with PHA (dark orange filled histogram) were stained with Mouse Anti-Human CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB23591A) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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