Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 298614
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108

Specificity

Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD69 PE‑conjugated Antibody

Detection of CD69 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB23591P) or isotype control antibody (IC003P, data not shown). View our protocol for Staining Membrane-associated Proteins.

Detection of CD69 in stimulated vs. naive PBMCs by Flow Cytometry.

PBMCs either treated with 5 ug/mL PHA for 2 days (A) or (B) left untreated (naive) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human CD69 PE‑conjugated Monoclonal Antibody (Catalog # FAB23591P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD69 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes; PBMCs treated with 5 ug/mL PHA for 2 days or left untreated

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Gene Symbol

CD69

UniProt

Q07108

Additional CD69 Products

Product Documents for Human CD69 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD69 PE‑conjugated Antibody

For research use only

Citations for Human CD69 PE‑conjugated Antibody

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