Human CD69 PE-conjugated Antibody

  (2 citations)     
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Product Details
Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 298614
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD69
    Gly64-Lys199
    Accession # Q07108
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 PE‑conjugated Monoclonal Antibody (Catalog # FAB23591P) or isotype control antibody (Catalog # IC003P, data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD69
CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.
  • Entrez Gene IDs:
    969 (Human); 12515 (Mouse)
  • Alternate Names:
    activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009;133(2):257-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 14  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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