Detection of CD94 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human CD94 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1058F) or (B) Mouse IgG1 Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD94 is a 30 kDa type II transmembrane protein with an extracellular C-type lectin domain. It is expressed by natural killer (NK) cells and a subset of CD8+ T cells, with cellular activation resulting in increased cell surface expression. Although CD94 can occur as a non-signaling homodimer, functional activity occurs when CD94 exists as a heterodimer with a NKG2 family member. The CD94/NKG2A heterodimer delivers an inhibitory signal to the expressing cell, whereas, the CD94/NKG2C heterodimer associates with the DAP12 adaptor protein and delivers an activating signal. Both heterodimeric complexes recognize HLA-E with an associated peptide derived from the signal peptide of other HLA proteins.
Entrez Gene IDs:
3824 (Human); 16643 (Mouse)
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor
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