Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CEACAM-8/CD66b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEACAM‑8/CD66b
Gln35-His141
Accession # P31997

Specificity

Detects human CEACAM‑8/CD66b in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-6, and rhCEACAM-7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CEACAM‑8/CD66b Antibody

Detection of CEACAM‑8/CD66b antibody in Human Granulocytes antibody by Flow Cytometry.

Detection of CEACAM‑8/CD66b in Human Granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-8/CD66b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Applications for Human CEACAM‑8/CD66b Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood granulocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human CEACAM‑8/CD66b

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-8/CD66b

CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Gene Symbol

CEACAM8

UniProt

P31997

Additional CEACAM-8/CD66b Products

Product Documents for Human CEACAM‑8/CD66b Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CEACAM‑8/CD66b Antibody

For research use only

Citations for Human CEACAM‑8/CD66b Antibody

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