Human CHD1L Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CHD1L in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) CHD1 and rhCHD5 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CHD1L
    Arg759-Lys879
    Accession # Q86WJ1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CHD1L by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CHD1L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1L at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHD1L

CHD-1L (Chromohelicase/ATPase DNA-binding protein 1-Like; also ALC-1) is a 98-118 kDa member of the SNF2/RAD54 helicase family of proteins. It is expressed in hepatocytes, possesses ATPase activity, and likely promotes chromatin remodeling at sites of DNA damage. It also is considered an oncogene. CHD-1L upregulates ARHGEF9 trabscription, an action that promotes Cdc42 activity with accompanying filopodia formation and EMT. It also binds apoptosis-mediating Nur77, blocking its migration from nucleus-to-mitochondria. Human CHD-1L is 897 amino acids (aa) in length. It contains a helicase ATP-binding domain (aa 58-223), a C-terminal helicase domain (aa 351-513), a coiled-coil region (aa 38-675) and one Macro domain that binds poly-ADP-ribose and targets DNA damage sites (aa 704-897). There are at least two Ser/Thr phosphorylation sites. There are multiple potential splice variants. One isoform contains an alternative start site at Met114, a second isoform possesses a deletion of aa 43-246, a third isoform shows a three aa substitution for aa 363-897, and a fourth isoform combines a 16 aa insertion after Arg386 with a five aa substitution for aa 425-897. Over aa 759-879, human CHD-1L shares 94% aa identity with mouse CHD-1L.

  • Long Name:
    Chromodomain Helicase DNA Binding Protein 1-like
  • Entrez Gene IDs:
    9557 (Human); 68058 (Mouse); 310707 (Rat)
  • Alternate Names:
    ALC1; amplified in liver cancer 1; amplified in liver cancer protein 1; CHD1L; CHDL; chromodomain helicase DNA binding protein 1-like; chromodomain-helicase-DNA-binding protein 1-like; FLJ22530
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