Human CIS-1 Antibody (989616)

R&D Systems | Catalog # MAB3194

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 989616
View all CIS-1 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human CIS-1
Leu11-Leu258
Accession # Q9NSE2

Specificity

Detects human CIS-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human CIS-1 Antibody (989616)

Detection of Human CIS-1 antibody by Western Blot.

Detection of Human CIS‑1 by Western Blot.

Western blot shows lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human CIS-1 Monoclonal Antibody (Catalog # MAB3194) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CIS-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human CIS-1 Antibody (989616)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human lung tissue and Human kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CIS-1

Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.

Long Name

Cytokine Inducible SH2-containing Protein

Alternate Names

CISH

Entrez Gene IDs

1154 (Human); 12700 (Mouse); 83681 (Rat)

Gene Symbol

CISH

UniProt

Q9NSE2

Additional CIS-1 Products

Product Documents for Human CIS-1 Antibody (989616)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CIS-1 Antibody (989616)

For research use only

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