Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.
Human Creatine Kinase MM/CKMM Antibody
R&D Systems | Catalog # G-113-C
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Creatine Kinase MM/CKMM Antibody
Detection of Human Creatine Kinase MM/CKMM by Simple WesternTM.
Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/CKMM at approximately 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Creatine Kinase MM/CKMM Antigen Affinity-purified Polyclonal Antibody (Catalog # G-113-C). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Applications for Human Creatine Kinase MM/CKMM Antibody
Application
Recommended Usage
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.
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Formulation
Lyophilized from a 0.2 μm filtered solution in NaH<SUB>2</SUB>PO<SUB>4</SUB>, NaCl and Sodium Azide with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Creatine Kinase MM/CKMM
Long Name
Creatine Kinase, Muscle
Alternate Names
CKM, CKMM, M-CK
Gene Symbol
CKM
Additional Creatine Kinase MM/CKMM Products
Product Documents for Human Creatine Kinase MM/CKMM Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Creatine Kinase MM/CKMM Antibody
For research use only
Related Research Areas
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