Key Product Details

Species Reactivity

Goat

Applications

Immunocytochemistry

Label

NL493 (Excitation = 493 nm, Emission = 514 nm)

Antibody Source

Polyclonal Donkey IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Goat IgG

Specificity

Goat IgG (H+L)

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG NorthernLights™ NL493-conjugated Antibody

Detection of Goat IgG Primary Antibody by Immunocytochemistry.

Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Goat IgG NorthernLights™ NL493-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Use at a 1:200 dilution

Reviewed Applications

Read 1 review rated 5 using NL003 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG NorthernLights™ NL493-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Goat IgG NorthernLights™ NL493-conjugated Antibody

For research use only

Citations for Goat IgG NorthernLights™ NL493-conjugated Antibody

Customer Reviews for Goat IgG NorthernLights™ NL493-conjugated Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Goat IgG NorthernLights™ NL493-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody
    Name: Hayk Simonyan
    Application: Immunohistochemistry
    Sample Tested: Mouse cortex
    Species: Mouse
    Verified Customer | Posted 06/23/2017
    Mouse cortex endothelial cells, 10um thickness sections of perfused brain. Dilution of secondary is 1:1000.
    Goat IgG NorthernLights™ NL493-conjugated Antibody NL003

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs for Goat IgG NorthernLights™ NL493-conjugated Antibody

Showing  1 - 1 of 1 FAQ Showing All

    • Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
      A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Secondary Antibodies