Goat IgG NorthernLights™ NL637-conjugated Antibody

NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL002

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Detection of Goat IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (5)
FAQs
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Goat IgG NorthernLights™ NL637-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Goat IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL637 (Excitation= 637 nm, Emission= 658 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Use at a 1:200 dilution
 
Immunohistochemistry
Use at a 1:200 dilution
 
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Goat IgG Primary Antibody by Immunocytochemistry. Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG NorthernLights™ NL637-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The abnormal accumulation of heparan sulfate in patients with mucopolysaccharidosis prevents the elastolytic activity of cathepsin V
    Authors: T Chazeirat, S Denamur, KK Bojarski, PM Andrault, D Sizaret, F Zhang, A Saidi, M Tardieu, RJ Linhardt, F Labarthe, D Brömme, SA Samsonov, G Lalmanach, F Lecaille
    Carbohydrate polymers, 2020-10-20;253(0):117261.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Developmental changes in myocardial B cells mirror changes in B cells associated with different organs
    Authors: C Rocha-Rese, W Yang, W Li, D Kreisel, L Adamo, DL Mann
    JCI Insight, 2020-08-20;5(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Efficient in Utero Gene Transfer to the Mammalian Inner Ears by the Synthetic Adeno-Associated Viral Vector Anc80L65
    Authors: CJ Hu, YC Lu, YH Tsai, HY Cheng, H Takeda, CY Huang, R Xiao, CJ Hsu, JW Tsai, LH Vandenberg, CC Wu, YF Cheng
    Mol Ther Methods Clin Dev, 2020-06-24;18(0):493-500.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Enhanced accumulation of N-terminally truncated A&beta with and without pyroglutamate-11 modification in parvalbumin-expressing GABAergic neurons in idiopathic and dup15q11.2-q13 autism
    Authors: J Frackowiak, B Mazur-Kole, P Mehta, J Wegiel
    Acta Neuropathol Commun, 2020-04-28;8(1):58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha.
    Authors: Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D
    Science, 2010-11-05;330(6005):827-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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