Donkey Anti-Goat IgG Antibody

Catalog # Availability Size / Price Qty
AF109
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Donkey Anti-Goat IgG Antibody Summary

Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains. In direct ELISAs, approximately 5% cross-reactivity with human IgG, mouse IgG, rabbit IgG and less than 1% cross-reactivity with chicken IgY is observed.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Donkey Anti-Goat IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Resveratrol attenuates triglyceride accumulation associated with upregulation of Sirt1 and lipoprotein lipase in 3T3-L1 adipocytes
    Authors: H Imamura, D Nagayama, N Ishihara, S Tanaka, R Watanabe, Y Watanabe, Y Sato, T Yamaguchi, N Ban, H Kawana, M Ohira, K Endo, A Saiki, K Shirai, I Tatsuno
    Mol Genet Metab Rep, 2017;12(0):44-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  2. Quantity and accessibility for specific targeting of receptors in tumours.
    Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
    Sci Rep, 2014;4(0):5232.
    Species: Human
    Sample Types: Whole Cells
    Applications: Not Specified
  3. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2007;40(5):1276-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl
  4. Osteoprotegerin (OPG)--a potential new role in the regulation of endothelial cell phenotype and tumour angiogenesis?
    Authors: Cross SS, Yang Z, Brown NJ, Balasubramanian SP, Evans CA, Woodward JK, Neville-Webbe HL, Lippitt JM, Reed MW, Coleman RE, Holen I
    Int. J. Cancer, 2006;118(8):1901-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. GITR, a member of the TNF receptor superfamily, is costimulatory to mouse T lymphocyte subpopulations.
    Authors: Ronchetti S, Zollo O, Bruscoli S, Agostini M, Bianchini R, Nocentini G, Ayroldi E, Riccardi C
    Eur. J. Immunol., 2004;34(3):613-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl
  6. Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands.
    Authors: Latchney LR, Fallon MA, Culp DJ, Gelbard HA, Dewhurst S
    J. Histochem. Cytochem., 2004;52(5):671-81.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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