Goat IgG (H+L) Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
F0109
Detection of Goat IgGPrimary Antibody by Flow Cytometry
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Product Details
Citations (2)
FAQs
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Goat IgG (H+L) Fluorescein-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Goat IgGPrimary Antibody by Flow Cytometry View Larger

Detection of Goat IgGPrimary Antibody by Flow Cytometry Human peripheral blood lymphocytes were stained with GoatAnti-Human Common  beta  Chain Antigen Affinity-purified PolyclonalAntibody (Catalog # AF906,filled histogram) or control antibody (Catalog #
AB-108-C
,open histogram), followed by Fluorescein-conjugated Anti-Goat IgG SecondaryAntibody (Catalog # F0109) at 10 uL/106 cells. 

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Goat IgG (H+L) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity.
    Authors: Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari N, Campana L, Capobianco A, Otten L, Particelli F, Acha-Orbea H, Baruffaldi F, Faccio R, Sitia R, Reith W, Cenci S
    J Bone Miner Res, 2014;29(2):290-303.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Secondary
  2. A porous photocurable elastomer for cell encapsulation and culture.
    Authors: Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R
    Biomaterials, 2007;28(32):4826-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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