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Human CLEC-1 Antibody

Catalog #: MAB17041 Datasheet / COA / SDS

Discontinued Product

MAB17041 has been discontinued.
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Detection of CLEC‑1 in Human Blood Monocytes by Flow Cytometry.
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Human CLEC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CLEC-2, -3B, -10A, or -14A is observed.
Source
Monoclonal Mouse IgG2B Clone # 685316
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-1
Gln77-Asp280
Accession # Q8NC01
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CLEC-1 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CLEC‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CLEC-1 Monoclonal Antibody (Catalog # MAB17041, filled histo-gram) or isotype control antibody (Catalog # MAB0041, open histo-gram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-1

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC-1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26%‑36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Pyz, E. et al. (2006) Ann. Med. 38:242.
  3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.
Long Name
C-type Lectin-like Receptor-1
Entrez Gene IDs
51267 (Human); 243653 (Mouse)
Alternate Names
CLEC1; CLEC-1; CLEC1A; CLEC1C-type lectin domain family 1 member A; C-type lectin domain family 1, member A; C-type lectin-like receptor 1; C-type lectin-like receptor-1; MGC34328

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