Human/Mouse/Rat GAPDH Antibody

Catalog # Availability Size / Price Qty
MAB5718
MAB5718-SP
Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot.
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Product Details
Citations (41)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat GAPDH Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat GAPDH/G3PDH in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse GAPDH/G3PDH and no cross-reactivity with recombinant human GAPDH-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 686613
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GAPDH/G3PDH
Met1-Ala150
Accession # P04406
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Simple Western
0.25 - 1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat GAPDH/G3PDH antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAPDH/G3PDH at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry GAPDH antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

GAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat GAPDH Monoclonal Antibody (Catalog # MAB5718) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry GAPDH/G3PDH antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse GAPDH/G3PDH antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse GAPDH/G3PDH by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH/G3PDH at approximately 41 kDa (as indicated) using 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog # MAB5718). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western View Larger

Detection of Human, Mouse, and Rat GAPDH by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 42 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH Monoclonal Antibody (Catalog # MAB5718). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAPDH

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a 36-40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length. There are two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151-155. GAPDH contains no fewer that 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over
aa 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Entrez Gene IDs
2597 (Human); 14433 (Mouse); 24383 (Rat)
Alternate Names
aging-associated gene 9 protein; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PDH; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH

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Citations for Human/Mouse/Rat GAPDH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL
    Authors: Liu WH, Mrozek-Gorska P, Wirth AK et al.
    Biomarker research
  2. AAV9-mediated FIG4 delivery prolongs life span in Charcot Marie Tooth disease type 4J mouse model
    Authors: Presa M, Bailey RM, Davis C et al.
    The Journal of clinical investigation
  3. Lamin B1 overexpression alters chromatin organization and gene expression
    Authors: Jeanae M. Kaneshiro, Juliana S. Capitanio, Martin W. Hetzer
    Nucleus
  4. Arachidin-1, a Prenylated Stilbenoid from Peanut, Enhances the Anticancer Effects of Paclitaxel in Triple-Negative Breast Cancer Cells
    Authors: S Mohammadho, A Weaver, M Sudhakaran, LC Ho, T Le, AI Doseff, F Medina-Bol
    Cancers, 2023-01-07;15(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Hyaluronic acid restored protein permeability across injured human lung microvascular endothelial cells
    Authors: Shinji Sugita, Yoshifumi Naito, Li Zhou, Hongli He, Qi Hao, Atsuhiro Sakamoto et al.
    FASEB BioAdvances
  6. Exosome-guided direct reprogramming of tumor-associated macrophages from protumorigenic to antitumorigenic to fight cancer
    Authors: H Kim, HJ Park, HW Chang, JH Back, SJ Lee, YE Park, EH Kim, Y Hong, G Kwak, IC Kwon, JE Lee, YS Lee, SY Kim, Y Yang, SH Kim
    Bioactive materials, 2022-08-05;25(0):527-540.
  7. Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model
    Authors: Mariko Sawa, Cassia Overk, Ann Becker, Dominique Derse, Ricardo Albay, Kim Weldy et al.
    Alzheimer's & Dementia
  8. G alpha 13 Mediates Transendothelial Migration of Neutrophils by Promoting Integrin-Dependent Motility without Affecting Directionality
    Authors: Claire W. Chang, Ni Cheng, Yanyan Bai, Randal A. Skidgel, Xiaoping Du
    The Journal of Immunology
  9. Dietary Intervention, When Not Associated With Exercise, Upregulates Irisin/FNDC5 While Reducing Visceral Adiposity Markers in Obese Rats
    Authors: Vanessa de Oliveira Furino, João Manoel Alves, Diego Adorna Marine, Marcela Sene-Fiorese, Carla Nascimento dos Santos Rodrigues, Cristina Arrais-Lima et al.
    Frontiers in Physiology
  10. An epigenetic switch regulates the ontogeny of AXL-positive/EGFR-TKi-resistant cells by modulating miR-335 expression
    Authors: Polona Safaric Tepes, Debjani Pal, Trine Lindsted, Ingrid Ibarra, Amaia Lujambio, Vilma Jimenez Sabinina et al.
    eLife
  11. Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy
    Authors: Stephen Gargan, Paul Dowling, Margit Zweyer, Jens Reimann, Michael Henry, Paula Meleady et al.
    Life (Basel)
  12. Functional Redundancy of Cyclase-Associated Proteins CAP1 and CAP2 in Differentiating Neurons
    Authors: F Schneider, I Metz, S Khudayberd, MB Rust
    Cells, 2021-06-17;10(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts
    Authors: LJ Kepser, S Khudayberd, LS Hinojosa, C Macchi, M Ruscica, E Marcello, C Culmsee, R Grosse, MB Rust
    Scientific Reports, 2021-02-26;11(1):4789.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Comparative Cell Surface Proteomic Analysis of the Primary Human T Cell and Monocyte Responses to Type I Interferon
    Authors: Lior Soday, Martin Potts, Leah M. Hunter, Benjamin J. Ravenhill, Jack W. Houghton, James C. Williamson et al.
    Frontiers in Immunology
  15. miR-146b Functions as an Oncogene in Oral Squamous Cell Carcinoma by Targeting HBP1
    Authors: Kui Li, Zheng Zhou, Ju Li, Rui Xiang
    Technol Cancer Res Treat
  16. Using chemiluminescence imaging of cells (CLIC) for relative protein quantification
    Authors: J Fisher, OE Sørensen, AHA Abu-Humaid
    Sci Rep, 2020-10-26;10(1):18280.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Uniaxial Static Strain Promotes Osteoblast Proliferation and Bone Matrix Formation in Distraction Osteogenesis In Vitro
    Authors: Z Li, J Zheng, D Wan, X Yang
    Biomed Res Int, 2020-08-12;2020(0):3906426.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Effect of sodium (S)-2-hydroxyglutarate in male, and succinic acid in female Wistar rats against renal ischemia-reperfusion injury, suggesting a role of the HIF-1 pathway
    Authors: E Cienfuegos, TR Ibarra-Riv, AL Saucedo, LA Ramírez-Ma, D Esquivel-F, I Domínguez-, KJ Alcántara-, DP Moreno-Peñ, G Alarcon-Ga, DR Rodríguez-, L Torres-Gon, LE Muñoz-Espi, E Pérez-Rodr, P Cordero-Pé
    PeerJ, 2020-07-10;8(0):e9438.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer
    Authors: G Kaufman, NM Kiburi, D Skrtic
    Biosci. Rep., 2020-06-26;40(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Interleukin‑22 regulates gastric cancer cell proliferation through regulation of the JNK signaling pathway
    Authors: Hao Dong, Fengming Zhu, Shilu Jin, Jing Tian
    Experimental and Therapeutic Medicine
  21. Identification of the Neuroinvasive Pathogen Host Target, LamR, as an Endothelial Receptor for the Treponema pallidum Adhesin Tp0751
    Authors: KV Lithgow, B Church, A Gomez, E Tsao, S Houston, LA Swayne, CE Cameron
    mSphere, 2020-04-01;5(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  22. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity
    Authors: Yeonsun Hong, Yoon Kyoung Kim, Gi Beom Kim, Gi-Hoon Nam, Seong A Kim, Yoon Park et al.
    Journal of Extracellular Vesicles
  23. A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice
    Authors: S Varshney, HX Wei, F Batista, M Nauman, S Sundaram, K Siminovitc, A Tanwar, P Stanley
    BMC Dev. Biol., 2019-10-07;19(1):19.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Western Blot
  24. Effects of Cariprazine, Aripiprazole, and Olanzapine on Mouse Fibroblast Culture: Changes in Adiponectin Contents in Supernatants, Triglyceride Accumulation, and Peroxisome Proliferator-Activated Receptor-gamma Expression
    Authors: László-István Bába, Melinda Kolcsár, Imre Zoltán Kun, Zsófia Ulakcsai, Fruzsina Bagaméry, Éva Szökő et al.
    Medicina (Kaunas)
  25. Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor
    Authors: K Watanabe, M Suzukawa, S Arakawa, K Kobayashi, S Igarashi, H Tashimo, H Nagai, S Tohma, T Nagase, K Ohta
    Allergol Int, 2019-04-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Metformin exhibited anticancer activity by lowering cellular cholesterol content in breast cancer cells
    Authors: A Sharma, S Bandyopadh, K Chowdhury, T Sharma, R Maheshwari, A Das, G Chakrabart, V Kumar, CC Mandal
    PLoS ONE, 2019-01-09;14(1):e0209435.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Geranylgeraniol (GGOH) as a Mevalonate Pathway Activator in the Rescue of Bone Cells Treated with Zoledronic Acid: An In Vitro Study
    Authors: RM Fliefel, SA Entekhabi, M Ehrenfeld, S Otto
    Stem Cells Int, 2019-01-09;2019(0):4351327.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  28. Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ beta -Catenin Pathway
    Authors: Yang Wang, Qi Yang, Yanli Cheng, Meng Gao, Lei Kuang, Chun Wang
    Medical Science Monitor
  29. High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms
    Authors: Katie Nightingale, Kai-Min Lin, Benjamin J. Ravenhill, Colin Davies, Luis Nobre, Ceri A. Fielding et al.
    Cell Host & Microbe
  30. Effects of 10 weeks of regular running exercise with and without parallel PDTC treatment on expression of genes encoding sarcomere-associated proteins in murine skeletal muscle
    Authors: Angelika Schmitt, Anne-Lena Haug, Franziska Schlegel, Annunziata Fragasso, Barbara Munz
    Cell Stress and Chaperones
  31. Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: The impact on host cells
    Authors: SY Ma'ayeh, J Liu, D Peirasmaki, K Hörnaeus, S Bergström, M Grabherr, J Bergquist, SG Svärd
    PLoS Negl Trop Dis, 2017-12-11;11(12):e0006120.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus
    Authors: Lijie Zhao, Tingli Sun, Lina Wang
    Therapeutics and Clinical Risk Management
  33. Whole-exome and targeted sequencing identify ROBO1 and ROBO2 mutations as progression-related drivers in myelodysplastic syndromes
    Authors: Feng Xu, Ling-Yun Wu, Chun-Kang Chang, Qi He, Zheng Zhang, Li Liu et al.
    Nature Communications
  34. Lactoferrin Promotes Early Neurodevelopment and Cognition in Postnatal Piglets by Upregulating the BDNF Signaling Pathway and Polysialylation
    Authors: Yue Chen, Zhiqiang Zheng, Xi Zhu, Yujie Shi, Dandan Tian, Fengjuan Zhao et al.
    Molecular Neurobiology
  35. The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells.
    Authors: Gutknecht M, Geiger J, Joas S, Dorfel D, Salih H, Muller M, Grunebach F, Rittig S
    Cell Commun Signal, 2015-03-24;13(0):19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Simultaneous silencing of multiple RB and p53 pathway members induces cell cycle reentry in intact human pancreatic islets
    Authors: Stanley Tamaki, Christopher Nye, Euan Slorach, David Scharp, Helen M Blau, Phyllis E Whiteley et al.
    BMC Biotechnology
  37. Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma.
    Authors: Kondo S, Mukudai Y, Soga D, Nishida T, Takigawa M, Shirota T
    Anticancer Res, 2014-02-01;34(2):671-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis.
    Authors: Liang G, Gao W, Liang A
    PLoS ONE, 2012-05-15;7(5):e36648.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. Intraluminal chloride regulates lung branching morphogenesis: involvement of PIEZO1/PIEZO2
    Authors: AN Gonçalves, RS Moura, J Correia-Pi, C Nogueira-S
    Respiratory Research, 2023-02-05;24(1):42.
  40. Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions
    Authors: Nobre LV, Nightingale K, Ravenhill BJ et al.
    Elife
  41. Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma.
    Authors: Zhou Yan-Wu, Li Rong, Duan Chao-Jun et al.
    Molecular Medicine Reports

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Human/Mouse/Rat GAPDH Antibody
By Lucy Quach on 07/03/2018
Application: WB Sample Tested: Cancer cell lysates,Xenograft Lysates Species: Human and Mouse

Human/Mouse/Rat GAPDH Antibody
By Anonymous on 03/01/2017
Application: WB Sample Tested: Human cell line Species: Human

Expression of Human p75NGF in several cell lines, with GAPDH levels showing equal loading. Dilution: 1:10,000 in PBS with 5% BSA. Secondary Ab: 1:5,000 anti-mouse IgG.