Human/Mouse/Rat GAPDH Antibody

Catalog # Availability Size / Price Qty
AF5718
AF5718-SP
Human/Mouse/Rat GAPDH Antibody in Western Blot
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Product Details
Citations (15)
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Human/Mouse/Rat GAPDH Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GAPDH/G3PDH
Met1-Ala150
Accession # P04406
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. View Larger

Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry GAPDH in HeLa Human Cell Line. View Larger

GAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry GAPDH/G3PDH in Human Kidney. View Larger

GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.

Immunohistochemistry GAPDH in Human Prostate Cancer Tissue. View Larger

GAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human GAPDH by Simple Western<sup>TM</sup>. View Larger

Detection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Entrez Gene IDs
2597 (Human); 14433 (Mouse); 24383 (Rat)
Alternate Names
aging-associated gene 9 protein; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PDH; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH

Product Datasheets

Citations for Human/Mouse/Rat GAPDH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey
    Authors: J Tan, X Wang, S Cai, F He, D Zhang, D Li, X Zhu, L Zhou, N Fan, X Liu
    Mol Ther Methods Clin Dev, 2020;17(0):143-155.
    Species: Human
    Sample Types: Cell
    Applications: Western Blot
  2. Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway
    Authors: A Der Vartan, J Chabanais, C Carrion, A Maftah, A Germot
    Int J Mol Sci, 2019;20(18):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism
    Authors: T Liu, NA Barrett, Y Kanaoka, K Buchheit, TM Laidlaw, D Garofalo, J Lai, HR Katz, C Feng, JA Boyce
    Mucosal Immunol, 2019;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  4. PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo
    Authors: L Liu, J Cao, J Zhao, X Li, Z Suo, H Li
    Onco Targets Ther, 2019;12(0):9899-9913.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium
    Authors: B Ilyas, DT Mulder, DJ Little, W Elhenawy, MM Banda, D Pérez-Mora, CN Tsai, NYE Chau, VH Bustamante, BK Coombes
    Cell Rep, 2018;25(4):825-832.e5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
    Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
    Cell. Physiol. Biochem., 2017;44(5):1896-1911.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
    Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
    Stem Cells, 2017;35(4):886-897.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy
    Authors: R Ganesan, NJ Hos, S Gutierrez, J Fischer, JM Stepek, E Daglidu, M Krönke, N Robinson
    PLoS Pathog, 2017;13(2):e1006227.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis
    Authors: MK Shim, HY Yoon, S Lee, MK Jo, J Park, JH Kim, SY Jeong, IC Kwon, K Kim
    Sci Rep, 2017;7(1):16635.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  10. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
    Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
    Sci Rep, 2017;7(1):5081.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
    Authors: Chen, Felicia, Marquez, Hector, Kim, Youn-Kyu, Qian, Jun, Shao, Fengzhi, Fine, Alan, Cruikshank, William, Quadro, Loredana, Cardoso, Wellingt
    J Clin Invest, 2014;124(2):801-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  12. Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
    Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
    Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
    Authors: Zhi, Hui, Luptak, Ivan, Alreja, Gaurav, Shi, Jianru, Guan, Jian, Metes-Kosik, Nicole, Joseph, Jacob
    PLoS ONE, 2013;8(12):e81612.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
    Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
    BMC Cancer, 2012;12(0):201.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours.
    Authors: Dungwa JV, Hunt LP, Ramani P
    BMC Cancer, 2011;11(0):390.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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