Human/Mouse/Rat GAPDH Antibody
Human/Mouse/Rat GAPDH Antibody Summary
Met1-Ala150
Accession # P04406
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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GAPDH in HeLa Human Cell Line. GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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GAPDH/G3PDH in Human Kidney. GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.
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GAPDH in Human Prostate Cancer Tissue. GAPDH was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of Human GAPDH by Simple WesternTM. Simple Western lane view shows lysates of human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAPDH
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.
Product Datasheets
Citations for Human/Mouse/Rat GAPDH Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
23
Citations: Showing 1 - 10
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Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice
Authors: CJ Chen, CC Wu, CY Chang, JR Li, YC Ou, WY Chen, SL Liao, JD Wang
International Journal of Molecular Sciences, 2022;23(16):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Western Blot -
Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
Cells, 2021;10(12):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy
Authors: TA Shah, HK Pallera, CL Kaszowski, WT Bass, FA Lattanzio
Frontiers in Neuroscience, 2021;15(0):616734.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Mouse WIF1 Is Only Modified with O-Fucose in Its EGF-like Domain III Despite Two Evolutionarily Conserved Consensus Sites
Authors: F Pennarubia, E Pinault, B Al Jaam, CE Brun, A Maftah, A Germot, S Legardinie
Biomolecules, 2020;10(9):.
Species: Hamster
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
Cancer Cell Int, 2020;20(0):476.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB
Authors: T Guerenne-D, V Couderc, L Duponchel, V Sol, P Leproux, JM Petit
Sci Rep, 2020;10(1):16749.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries
Authors: J Tan, X Zhang, D Li, G Liu, Y Wang, D Zhang, X Wang, W Tian, X Dong, L Zhou, X Zhu, X Liu, N Fan
Mol Ther Methods Clin Dev, 2020;17(0):894-903.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey
Authors: J Tan, X Wang, S Cai, F He, D Zhang, D Li, X Zhu, L Zhou, N Fan, X Liu
Mol Ther Methods Clin Dev, 2020;17(0):143-155.
Species: Human
Sample Types: Cell
Applications: Western Blot -
Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway
Authors: A Der Vartan, J Chabanais, C Carrion, A Maftah, A Germot
Int J Mol Sci, 2019;20(18):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms
Authors: MS Abdelfatta, MIY Elmallah, HY Ebrahim, RS Almeer, RMA Eltanany, AE Abdel Mone
PLoS ONE, 2019;14(6):e0216737.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo
Authors: L Liu, J Cao, J Zhao, X Li, Z Suo, H Li
Onco Targets Ther, 2019;12(0):9899-9913.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism
Authors: T Liu, NA Barrett, Y Kanaoka, K Buchheit, TM Laidlaw, D Garofalo, J Lai, HR Katz, C Feng, JA Boyce
Mucosal Immunol, 2019;0(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium
Authors: B Ilyas, DT Mulder, DJ Little, W Elhenawy, MM Banda, D Pérez-Mora, CN Tsai, NYE Chau, VH Bustamante, BK Coombes
Cell Rep, 2018;25(4):825-832.e5.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
Cell. Physiol. Biochem., 2017;44(5):1896-1911.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
Sci Rep, 2017;7(1):5081.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis
Authors: MK Shim, HY Yoon, S Lee, MK Jo, J Park, JH Kim, SY Jeong, IC Kwon, K Kim
Sci Rep, 2017;7(1):16635.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy
Authors: R Ganesan, NJ Hos, S Gutierrez, J Fischer, JM Stepek, E Daglidu, M Krönke, N Robinson
PLoS Pathog, 2017;13(2):e1006227.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells
Authors: CT Yang, R Ma, RA Axton, M Jackson, AH Taylor, A Fidanza, L Marenah, J Frayne, JC Mountford, LM Forrester
Stem Cells, 2017;35(4):886-897.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy.
Authors: Wang L, Shi P, Xu Z, Li J, Xie Y, Mitton K, Drenser K, Yan Q
Invest Ophthalmol Vis Sci, 2014;55(7):4276-87.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice.
Authors: Chen, Felicia, Marquez, Hector, Kim, Youn-Kyu, Qian, Jun, Shao, Fengzhi, Fine, Alan, Cruikshank, William, Quadro, Loredana, Cardoso, Wellingt
J Clin Invest, 2014;124(2):801-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function.
Authors: Zhi, Hui, Luptak, Ivan, Alreja, Gaurav, Shi, Jianru, Guan, Jian, Metes-Kosik, Nicole, Joseph, Jacob
PLoS ONE, 2013;8(12):e81612.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
BMC Cancer, 2012;12(0):201.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours.
Authors: Dungwa JV, Hunt LP, Ramani P
BMC Cancer, 2011;11(0):390.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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The blot contains HeLa cell lysate (1, 2, 3 and 5 µg) spiked with 200 ng of human transferrin per lane. Transferrin is detected in the red channel, tubulin is detected in the green channel and GAPDH is detected in the blue channel.
