Human Collagen XXIII  alpha 1 Antibody

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Human Collagen XXIII  alpha 1 Antibody in Data
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Human Collagen XXIII  alpha 1 Antibody Summary

Species Reactivity
Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human COL25A1 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Collagen XXIII alpha 1
Accession # Q86Y22
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Collagen XXIII a1 antibody by Western Blot. View Larger

Detection of Human Collagen XXIII alpha 1 by Western Blot. Western blot shows lysates of human alzheimer brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Collagen XXIII a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4165) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Collagen XXIII a1 at approximately 45 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XXIII alpha 1

Collagen XXIII alpha 1 (sometimes abbreviated COL23A1) is a ~75 kDa type II transmembrane nonfibrillar collagen that is a member of the collagenous transmembrane protein superfamily (1, 2). This family also includes collagens XIII, XVII, XXV and non‑collagens with triple‑helical regions such as ectodysplasin A, class A macrophage scavenger receptors, and MARCO (2). The human Collagen XXIII mRNA encodes a 540 amino acid (aa) protein containing a 34 aa N‑terminal cytoplasmic domain, a 21 aa transmembrane (TM) domain and a 485 aa extracellular domain (ECD). The ECD contains a coiled‑coil consensus sequence to aid homotrimerization (aa 64‑69), a furin cleavage site (aa 105‑110), a pair of cysteines thought to form intermolecular disulfides (aa 106 and 108), and three collagen domains (1, 3‑5). The C‑terminal 20 aa, including cysteines at aa 525 and 537 of Collagen XXIII, is conserved among TM collagen proteins. Proteolytic cleavage, occurring mainly in the golgi, allows the Collagen XXIII ectodomain to be secreted as a soluble trimer of ~60 kDa subunits (1, 5). Cell surface cleavage can also occur but is slow, presumably due to presence of Collagen XXIII in lipid raft membrane domains (5). The protein database includes three variants of 537, 316 and 309 aa with various portions missing or substituted; all appear to lack TM segments (6). The human Collagen XXIII ECD shares 92%, 93%, 85%, 85% and 84% aa identity with mouse, rat, canine, equine and bovine Collagen XXIII, respectively. Collagen XXIII is concentrated at sites of cell contact in epithelia, and is thought to be an adhesion molecule (2, 4). Its up‑regulation has been correlated with aggressiveness in transformed cells, particularly in prostate cancer (1, 7).

  1. Banyard, J. et al. (2003) J. Biol. Chem. 278:20989.
  2. Franzke, C-W. et al. (2005) J. Biol. Chem. 280:4005.
  3. Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
  4. Koch, M. et al. (2006) J. Biol. Chem. 281:21546.
  5. Veit, G. et al. (2007) J. Biol. Chem. 282:27424.
  6. Entrez Accession # EAW53833, EAW53834, and AAH42428.
  7. Banyard, J. et al. (2007) Clin. Cancer Res. 13:2634.
Entrez Gene IDs
91522 (Human); 237759 (Mouse); 353303 (Rat)
Alternate Names
COL23A1; collagen alpha-1(XXIII) chain; Collagen XXIII alpha 1; collagen, type XXIII, alpha 1; DKFZp434K0621; procollagen, type XXIII, alpha 1

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