Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.
Monoclonal Mouse IgG1 Clone # 81506
Protein A or G purified from hybridoma culture supernatant
NS0-derived and E. coli-derived Recombinant Human CX3CL1/Fracktalkine Gln25-Arg339 Accession # P78423
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Human CX3CL1/Fractalkine Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)
ELISA Detection (Matched Antibody Pair)
Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog # BAM365)
Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)
Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CX3CL1, also known as Fractalkine, is a type I membrane protein in
which a chemokine domain possessing a unique C-X3-C cysteine motif is
tethered on a long mucin-like stalk. It can also be released as a
soluble molecule upon proteolysis at a membrane proximal site.
Entrez Gene IDs:
6376 (Human); 20312 (Mouse); 89808 (Rat)
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.