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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sensitivity
    47 pg/mL
  • Assay Range
    156.00 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma,)
  • Specificity
    Natural and recombinant SDF-1 alpha
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested on one plate to assess intra-assay precision.
Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4911700342054416003150
Standard Deviation195811573150257
CV%3.93.43.413.49.48.2

Recovery

The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-108
Platelet-poor EDTA Plasma (n=5) 97 90-107
Platelet-poor Heparin Plasma (n=5) 97 90-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
Background: CXCL12/SDF-1 alpha
CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.
    • Entrez Gene IDs
      6387 (Human); 20315 (Human); 493806 (Feline);
    • Alternate Names
      CXCL12 chemokine (C-X-C motif) ligand 12; IRH; PBSF; SCYB12; SDF1; SDF1A; SDF1B; TLSF; TPAR1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 48
    Filter your results:

    Species
    Sample Type
    1. Intranasal delivery of mesenchymal stem cells significantly extends survival of irradiated mice with experimental brain tumors.
      Authors: Balyasnikova I, Prasol M, Ferguson S, Han Y, Ahmed A, Gutova M, Tobias A, Mustafi D, Rincon E, Zhang L, Aboody K, Lesniak M
      Mol Ther, 2013;22(1):140-8.
      Species: Human
      Sample Type: Cell Lysates
    2. Altered features and increased chemosensitivity of human breast cancer cells mediated by adipose tissue-derived mesenchymal stromal cells.
      Authors: Kucerova L, Skolekova S, Matuskova M, Bohac M, Kozovska Z
      BMC Cancer, 2013;13(0):535.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Breast milk from Tanzanian women has divergent effects on cell-free and cell-associated HIV-1 infection in vitro.
      Authors: Lyimo M, Mosi M, Housman M, Zain-Ul-Abideen M, Lee F, Howell A, Connor R
      PLoS ONE, 2012;7(8):e43815.
      Species: Human
      Sample Type: Milk
    4. Nodal lymphangiogenesis and metastasis: Role of tumor-induced lymphatic vessel activation in extramammary Paget's disease.
      Authors: Hirakawa S, Detmar M, Kerjaschki D, Nagamatsu S, Matsuo K, Tanemura A, Kamata N, Higashikawa K, Okazaki H, Kameda K, Nishida-Fukuda H, Mori H, Hanakawa Y, Sayama K, Shirakata Y, Tohyama M, Tokumaru S, Katayama I, Hashimoto K
      Am. J. Pathol., 2009;175(5):2235-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs.
      Authors: Makam M, Diaz D, Laval J, Gernez Y, Conrad CK, Dunn CE, Davies ZA, Moss RB, Herzenberg LA, Herzenberg LA, Tirouvanziam R
      Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5779-83.
    6. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
      Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C
      Blood, 2002;99(3):792-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Targeted disruption of the CXCL12/CXCR4 axis inhibits osteolysis in a murine model of myeloma-associated bone loss.
      Authors: Diamond P, Labrinidis A, Martin SK, Farrugia AN, Gronthos S, To LB, Fujii N, O'Loughlin PD, Evdokiou A, Zannettino AC
      J. Bone Miner. Res., 2009;24(7):1150-61.
    8. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
      Authors: Irhimeh MR, Fitton JH, Lowenthal RM
      Exp. Hematol., 2007;35(6):989-94.
    9. Vitreous levels of vascular endothelial growth factor and stromal-derived factor 1 in patients with diabetic retinopathy and cystoid macular edema before and after intraocular injection of triamcinolone.
      Authors: Brooks HL, Caballero S, Newell CK, Steinmetz RL, Watson D, Segal MS, Harrison JK, Scott EW, Grant MB
      Arch. Ophthalmol., 2004;122(12):1801-7.
      Species: Human
      Sample Type: Vitreous Humor
    10. Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction.
      Authors: Massa M, Rosti V, Ferrario M, Campanelli R, Ramajoli I, Rosso R, De Ferrari GM, Ferlini M, Goffredo L, Bertoletti A, Klersy C, Pecci A, Moratti R, Tavazzi L
      Blood, 2004;105(1):199-206.
      Species: Human
      Sample Type: Plasma
    11. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
      Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
      J. Orthop. Res., 2005;23(1):203-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. SDF-1 is both necessary and sufficient to promote proliferative retinopathy.
      Authors: Butler JM, Guthrie SM, Koc M, Afzal A, Caballero S, Brooks HL, Mames RN, Segal MS, Grant MB, Scott EW
      J. Clin. Invest., 2005;115(1):86-93.
      Species: Human
      Sample Type: Vitreous Humor
    13. Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment.
      Authors: Otsuka H, Arimura N, Sonoda S
      Am. J. Pathol., 2010;177(5):2268-77.
      Species: Human
      Sample Type: Vitreous Humor
    14. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
      Authors: Tso GH, Law HK, Tu W
      Stem Cells, 2010;28(5):939-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
      Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
      Mediators Inflamm., 2009;2009(0):269541.
      Species: Human
      Sample Type: Serum
    16. Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study.
      Authors: Zhu AX, Sahani DV, Duda DG, di Tomaso E, Ancukiewicz M, Catalano OA, Sindhwani V, Blaszkowsky LS, Yoon SS, Lahdenranta J, Bhargava P, Meyerhardt J, Clark JW, Kwak EL, Hezel AF, Miksad R, Abrams TA, Enzinger PC, Fuchs CS, Ryan DP, Jain RK
      J. Clin. Oncol., 2009;27(18):3027-35.
      Species: Human
      Sample Type: Plasma
    17. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
      Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
      Blood, 2011;117(13):3485-93.
    18. Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
      Authors: Quesnel C, Piednoir P, Gelly J, Nardelli L, Garnier M, Lecon V, Lasocki S, Bouadma L, Philip I, Elbim C, Mentre F, Soler P, Crestani B, Dehoux M
      Crit. Care Med., 2012;40(1):21-8.
      Species: Human
      Sample Type: BALF
    19. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex.
      Authors: Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ, Kucia M, Janowska-Wieczorek A, Ratajczak J
      Leukemia, 2010;24(5):976-85.
      Species: Human
      Sample Type: Plasma
    20. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
      Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
      Cancer Cell, 2008;14(3):263-73.
    21. Distraction osteogenesis induces endothelial progenitor cell mobilization without inflammatory response in man.
      Authors: Lee DY, Cho TJ, Lee HR, Park MS, Yoo WJ, Chung CY, Choi IH
      Bone, 2010;46(3):673-9.
      Species: Human
      Sample Type: Plasma
    22. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
      Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
      Cancer Res., 2009;69(15):6249-55.
    23. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
    24. Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
      Authors: Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N
      PLoS ONE, 2012;7(8):e43665.
      Species: Human
      Sample Type: Tissue Homogenates
    25. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
      Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
      Leuk. Lymphoma, 2006;47(6):1034-40.
      Species: Human
      Sample Type: Serum
    26. BMP9 regulates endoglin-dependent chemokine responses in endothelial cells.
      Authors: Young, Kira, Conley, Barbara, Romero, Diana, Tweedie, Eric, O'Neill, Christin, Pinz, Ilka, Brogan, Louise, Lindner, Volkhard, Liaw, Lucy, Vary, Calvin P
      Blood, 2012;120(20):4263-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
      Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
      Cancer Cell, 2009;16(4):309-23.
    28. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
      Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
      Eur. J. Immunol., 2004;34(4):1164-74.
      Species: Human
      Sample Type: Plasma
    29. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
      Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
      Cardiovasc. Res., 2011;89(1):157-65.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
      Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
      Biochem. Biophys. Res. Commun., 2007;363(3):472-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    31. Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
      Authors: Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H
      Cardiovasc. Res., 2006;71(3):455-65.
      Species: Rabbit
      Sample Type: Serum
    32. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
      Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
      J. Clin. Oncol., 2007;25(29):4542-9.
      Species: Human
      Sample Type: Plasma
    33. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
      Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
      J. Exp. Med., 2004;199(9):1213-21.
      Species: Human
      Sample Type: Cell Culture Supernates
    34. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
      Authors: Casazza S, Zappia E, Pistorio A, Gambini C
      Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
      Species: Human
      Sample Type: CSF
    35. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
      Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
      J. Leukoc. Biol., 2003;73(5):584-90.
      Species: Human
      Sample Type: CSF
    36. Role of the CXCL12/CXCR4 axis in peritoneal carcinomatosis of gastric cancer.
      Authors: Yasumoto K, Koizumi K, Kawashima A, Saitoh Y, Arita Y, Shinohara K, Minami T, Nakayama T, Sakurai H, Takahashi Y, Yoshie O, Saiki I
      Cancer Res., 2006;66(4):2181-7.
      Species: Human
      Sample Type: Ascites
    37. Glioblastoma and endothelial cells cross-talk, mediated by SDF-1, enhances tumour invasion and endothelial proliferation by increasing expression of cathepsins B, S, and MMP-9.
      Authors: Kenig S, Alonso MB, Mueller MM, Lah TT
      Cancer Lett., 2010;289(1):53-61.
    38. Phase I and pharmacokinetic study of sunitinib in pediatric patients with refractory solid tumors: a children's oncology group study.
      Authors: Dubois SG, Shusterman S, Ingle AM, Ahern CH, Reid JM, Wu B, Baruchel S, Glade-Bender J, Ivy P, Grier HE, Adamson PC, Blaney SM
      Clin. Cancer Res., 2011;17(15):5113-22.
      Species: Human
      Sample Type: Plasma
    39. Tissue inhibitor of metalloproteinase-3 via oncolytic herpesvirus inhibits tumor growth and vascular progenitors.
      Authors: Mahller YY, Vaikunth SS, Ripberger MC, Baird WH, Saeki Y, Cancelas JA, Crombleholme TM, Cripe TP
      Cancer Res., 2008;68(4):1170-9.
      Species: Human
      Sample Type: Plasma
    40. Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
      Authors: Lund TC, Stadem PS, Panoskaltsis-Mortari A, Raymond G, Miller WP, Tolar J, Orchard PJ
      PLoS ONE, 2012;7(2):e32218.
    41. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
      Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
      Cancer Res., 2012;72(1):66-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    42. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    43. Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
      Authors: Gyongyosi M, Posa A, Pavo N, Hemetsberger R, Kvakan H, Steiner-Boker S, Petrasi Z, Manczur F, Pavo IJ, Edes IF, Wojta J, Glogar D, Huber K
      Thromb. Haemost., 2010;104(2):376-84.
      Species: Human
      Sample Type: Plasma
    44. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
      Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
      Obesity (Silver Spring), 2011;19(11):2130-6.
      Species: Human
      Sample Type: Plasma
    45. Levels of circulating CD45(dim)CD34(+)VEGFR2(+) progenitor cells correlate with outcome in metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors.
      Authors: Farace F, Gross-Goupil M, Tournay E, Taylor M, Vimond N, Jacques N, Billiot F, Mauguen A, Hill C, Escudier B
      Br. J. Cancer, 2011;104(7):1144-50.
      Species: Human
      Sample Type: Plasma
    46. Dysregulation of stromal derived factor 1/CXCR4 axis in the megakaryocytic lineage in essential thrombocythemia.
      Authors: Salim JP, Goette NP, Lev PR, Chazarreta CD, Heller PG, Alvarez C, Molinas FC, Marta RF
      Br. J. Haematol., 2009;144(1):69-77.
      Species: Human
      Sample Type: Plasma
    47. Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium.
      Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW
      Cancer Res., 2007;67(9):4244-53.
    48. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
      Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
      J. Immunol., 2008;181(11):8053-67.
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