Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

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CXCL12/SDF-1 alpha ELISA Kit ELISA
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Citations (101)
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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sensitivity
47 pg/mL
Assay Range
156.0 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity
Natural and recombinant SDF-1 alpha
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 491 1700 3420 544 1600 3150
Standard Deviation 19 58 115 73 150 257
CV% 3.9 3.4 3.4 13.4 9.4 8.2

Recovery

The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-108
Platelet-poor EDTA Plasma (n=5) 97 90-107
Platelet-poor Heparin Plasma (n=5) 97 90-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL12/SDF-1 alpha ELISA Linearity

Data Examples

Human CXCL12/SDF-1 alpha ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Entrez Gene IDs:
6387 (Human); 20315 (Mouse); 493806 (Feline)
Alternate Names:
CXCL12 chemokine (C-X-C motif) ligand 12; CXCL12/SDF-1 alpha; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

101 Citations: Showing 1 - 10
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  1. Assessment of agonistic and antagonistic properties of widely used oral care antimicrobial substances toward steroid estrogenic and androgenic receptors
    Authors: K Michalíkov, L Linhartová, M Ezechiáš, T Cajthaml
    Chemosphere, 2019;217(0):534-541.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
    Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
    Cell Transplant, 2019;0(0):9636897198256.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Pro-angiogenic impact of SDF-1? gene-activated collagen-based scaffolds in stem cell driven angiogenesis
    Authors: AL Laiva, RM Raftery, MB Keogh, FJ O'Brien
    Int J Pharm, 2018;544(2):372-379.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. miR-10a rejuvenates aged human mesenchymal stem cells and improves heart function after myocardial infarction through KLF4
    Authors: J Dong, Z Zhang, H Huang, P Mo, C Cheng, J Liu, W Huang, C Tian, C Zhang, J Li
    Stem Cell Res Ther, 2018;9(1):151.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin increases the activation of aryl hydrocarbon receptor and is associated with the aggressiveness of osteosarcoma MG-63 osteoblast-like cells
    Authors: SC Yang, CH Wu, YK Tu, SY Huang, PC Chou
    Oncol Lett, 2018;16(3):3849-3857.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Mobilization of progenitor cells and assessment of vessel healing after second generation drug-eluting stenting by optical coherence tomography
    Authors: M Sakuma, T Nasuno, S Abe, S Obi, S Toyoda, I Taguchi, R Sohma, KI Inoue, S Nishino, K Node, G Attizzani, H Bezerra, M Costa, D Simon, T Inoue
    Int J Cardiol Heart Vasc, 2018;18(0):17-24.
    Species: Human
    Sample Types: Serum
  7. Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-?B signaling pathway in vivo and in vitro
    Authors: B Liu, F Ding, D Hu, Y Zhou, C Long, L Shen, Y Zhang, D Zhang, G Wei
    Stem Cell Res Ther, 2018;9(1):7.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. The homing of bone marrow stem cells is differentially activated in ischemic and valvular heart�diseases and influenced by beta-blockers
    Authors: M Kristochec, LD Dias, C Ghem, B Eibel, RAK Kalil, MM Markoski
    J Transl Med, 2018;16(1):133.
    Species: Human
    Sample Types: Plasma
  9. The brain-penetrating CXCR4 antagonist, PRX177561, increases the antitumor effects of bevacizumab and sunitinib in preclinical models of human glioblastoma
    Authors: GL Gravina, A Mancini, F Marampon, A Colapietro, S Delle Mona, R Sferra, F Vitale, PJ Richardson, L Patient, S Burbidge, C Festuccia
    J Hematol Oncol, 2017;10(1):5.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli
    Authors: P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti
    Front Immunol, 2017;8(0):141.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Synovial inflammation plays a greater role in post-traumatic osteoarthritis compared to idiopathic osteoarthritis in the Hartley guinea pig knee
    Authors: NP Thomas, WJ Wu, BC Fleming, F Wei, Q Chen, L Wei
    BMC Musculoskelet Disord, 2017;18(1):556.
    Species: Guinea Pig
    Sample Types: Synovial Lavage Fluid
  12. The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical models
    Authors: GL Gravina, A Mancini, A Colapietro, F Vitale, A Vetuschi, S Pompili, G Rossi, F Marampon, PJ Richardson, L Patient, L Patient, S Burbidge, C Festuccia
    Tumour Biol., 2017;39(6):1010428317695.
    Species: Human
    Sample Types: Cell Lysates
  13. Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in�vitro
    Authors: Y Wang, S Lai, J Tang, C Feng, F Liu, C Su, W Zou, H Chen, D Xu
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Beneficial Effects of Reconstituted High-Density Lipoprotein (rHDL) on Circulating CD34+ Cells in Patients after an Acute Coronary Syndrome
    Authors: C Gebhard, E Rh‚aume, C Berry, G Brand, AE Kernalegue, G Th‚berge-J, MA Alam, CY Lee, L Boileau, M Chabot-Bla, MC Guertin, MA Lavoie, J Gr‚goire, R Ibrahim, P L'Allier, JC Tardif
    PLoS ONE, 2017;12(1):e0168448.
    Species: Human
    Sample Types: Serum
  15. Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells
    Authors: AK Batsali, C Pontikoglo, D Koutroulak, KI Pavlaki, A Damianaki, I Mavroudi, K Alpantaki, E Kouvidi, G Kontakis, HA Papadaki
    Stem Cell Res Ther, 2017;8(1):102.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis
    Authors: L Desbourdes, J Javary, T Charbonnie, N Ishac, J Bourgeais, A Iltis, JC Chomel, A Turhan, F Guilloton, K Tarte, MV Demattei, E Ducrocq, F Rouleux-Bo, E Gyan, O Hérault, J Domenech
    Stem Cells Dev., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Chemokine signals are crucial for enhanced homing and differentiation of circulating osteoclast progenitor cells
    Authors: A Sucur, Z Jajic, M Artukovic, MI Matijasevi, B Anic, D Flegar, A Markotic, T Kelava, S Ivcevic, N Kovacic, V Katavic, D Grcevic
    Arthritis Res. Ther., 2017;19(1):142.
    Species: Human
    Sample Types: Plasma
  18. Vildagliptin, but not glibenclamide, increases circulating endothelial progenitor cell number: a 12-month randomized controlled trial in patients with type 2 diabetes
    Authors: A Dei Cas, V Spigoni, M Cito, R Aldigeri, V Ridolfi, E Marchesi, M Marina, E Derlindati, R Aloe, RC Bonadonna, I Zavaroni
    Cardiovasc Diabetol, 2017;16(1):27.
    Species: Human
    Sample Types: Plasma
  19. Evaluation of autologous skeletal muscle-derived factors for regenerative medicine applications
    Authors: M Yoshikawa, T Nakasa, M Ishikawa, N Adachi, M Ochi
    Bone Joint Res, 2017;6(5):277-283.
    Species: Human
    Sample Types: Tissue Homogenates
  20. Angiogenic T cell expansion correlates with severity of peripheral vascular damage in systemic sclerosis
    Authors: M Manetti, S Pratesi, E Romano, S Bellando-R, I Rosa, S Guiducci, BS Fioretto, L Ibba-Manne, E Maggi, M Matucci-Ce
    PLoS ONE, 2017;12(8):e0183102.
    Species: Human
    Sample Types: Serum
  21. A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
    Authors: DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson
    PLoS ONE, 2017;12(4):e0174447.
  22. Tadalafil, a long acting phosphodiesterase inhibitor, promotes bone marrow stem cell survival and their homing into ischemic myocardium for cardiac repair
    Authors: I Elmadbouh, M Ashraf
    Physiol Rep, 2017;5(21):.
    Species: Human
    Sample Types: Cell Lysates
  23. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    PLoS ONE, 2016;11(12):e0168058.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Kinetics of circulating endothelial progenitor cells in patients undergoing carotid artery surgery
    Authors: Günay Kalender
    Ther Clin Risk Manag, 2016;12(0):1841-1847.
    Species: Human
    Sample Types: Plasma
  25. Early pancreatic cancer lesions suppress pain through CXCL12-mediated chemoattraction of Schwann cells
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  26. Circulating Fibrocytes Are Increased in Neonates with Bronchopulmonary Dysplasia
    Authors: Chun Li
    PLoS ONE, 2016;11(6):e0157181.
    Species: Human
    Sample Types: Plasma
  27. Characterizing the angiogenic activity of patients with single ventricle physiology and aortopulmonary collateral vessels
    Authors: N Sandeep, Y Uchida, K Ratnayaka, R McCarter, S Hanumantha, A Bangoura, Z Zhao, J Oliver-Dan, L Leatherbur, J Kanter, YS Mukouyama
    J. Thorac. Cardiovasc. Surg., 2016;151(4):1126-35.e2.
    Species: Human
    Sample Types: Plasma
  28. BH3 mimetics suppress CXCL12 expression in human malignant peripheral nerve sheath tumor cells
    Authors: CD Graham, N Kaza, HC Pruitt, LM Gibson, BJ Klocke, LA Shevde, SL Carroll, KA Roth
    Oncotarget, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. High throughput chemical library screening identifies a novel p110-? inhibitor that potentiates the anti-myeloma effect of bortezomib
    Oncotarget, 2016;7(25):38523-38538.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016;11(8):e0160153.
    Species: Human
    Sample Types: Plasma
  32. Ligand-activated PPAR? downregulates CXCR4 gene expression through a novel identified PPAR response element and inhibits breast cancer progression
    Authors: D Rovito, G Gionfriddo, I Barone, C Giordano, F Grande, F De Amicis, M Lanzino, S Catalano, S Andò, D Bonofiglio
    Oncotarget, 2016;7(40):65109-65124.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Improved tumor vascularization after anti-VEGF therapy with carboplatin and nab-paclitaxel associates with survival in lung cancer.
    Authors: Heist, Rebecca, Duda, Dan G, Sahani, Dushyant, Ancukiewicz, Marek, Fidias, Panos, Sequist, Lecia V, Temel, Jennifer, Shaw, Alice T, Pennell, Nathan A, Neal, Joel W, Gandhi, Leena, Lynch, Thomas J, Engelman, Jeffrey, Jain, Rakesh K
    Proc Natl Acad Sci U S A, 2015;112(5):1547-52.
    Species: Human
    Sample Types: Plasma
  34. Chronic hyperglycemia induces trans-differentiation of human pancreatic stellate cells and enhances the malignant molecular communication with human pancreatic cancer cells.
    Authors: Kiss K, Baghy K, Spisak S, Szanyi S, Tulassay Z, Zalatnai A, Lohr J, Jesenofsky R, Kovalszky I, Firneisz G
    PLoS ONE, 2015;10(5):e0128059.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4.
    Authors: Walsh T, Harper M, Poole A
    Cell Signal, 2015;27(1):37-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Elevated plasma CXCL12alpha is associated with a poorer prognosis in pulmonary arterial hypertension.
    Authors: McCullagh B, Costello C, Li L, O'Connell C, Codd M, Lawrie A, Morton A, Kiely D, Condliffe R, Elliot C, McLoughlin P, Gaine S
    PLoS ONE, 2015;10(4):e0123709.
    Species: Human
    Sample Types: Plasma
  37. Remodeling of extracellular matrix by normal and tumor-associated fibroblasts promotes cervical cancer progression.
    Authors: Fullar A, Dudas J, Olah L, Hollosi P, Papp Z, Sobel G, Karaszi K, Paku S, Baghy K, Kovalszky I
    BMC Cancer, 2015;15(0):256.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Transmissible cytotoxicity of multiple myeloma cells by cord blood-derived NK cells is mediated by vesicle trafficking.
    Authors: Martin-Antonio B, Najjar A, Robinson S, Chew C, Li S, Yvon E, Thomas M, Mc Niece I, Orlowski R, Munoz-Pinedo C, Bueno C, Menendez P, Fernandez de Larrea C, Urbano-Ispizua A, Shpall E, Shah N
    Cell Death Differ, 2015;22(1):96-107.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance.
    Authors: Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S
    Stem Cells, 2015;33(9):2838-49.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Detailed characterization of multiple myeloma circulating tumor cells shows unique phenotypic, cytogenetic, functional, and circadian distribution profile.
    Authors: Paiva B, Paino T, Sayagues J, Garayoa M, San-Segundo L, Martin M, Mota I, Sanchez M, Barcena P, Aires-Mejia I, Corchete L, Jimenez C, Garcia-Sanz R, Gutierrez N, Ocio E, Mateos M, Vidriales M, Orfao A, San Miguel J
    Blood, 2015;122(22):3591-8.
    Species: Human
    Sample Types: Plasma
  41. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    , 2014;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2014;123(7):1032-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Effects of pitavastatin versus atorvastatin on the peripheral endothelial progenitor cells and vascular endothelial growth factor in high-risk patients: a pilot prospective, double-blind, randomized study.
    Authors: Lin L, Huang C, Chen J, Wu T, Leu H, Huang P, Chang T, Lin S, Chen J
    Cardiovasc Diabetol, 2014;13(0):111.
    Species: Human
    Sample Types: Serum
  44. Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression.
    Authors: Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J
    Placenta, 2014;35(12):1089-94.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
    Authors: Yu, Yue, Lee, Jennifer, Xie, Ning, Li, Estelle, Hurtado-Coll, Antonio, Fazli, Ladan, Cox, Michael, Plymate, Stephen, Gleave, Martin, Dong, Xuesen
    PLoS ONE, 2014;9(3):e92714.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Expression of and transcript variants and in epithelial ovarian cancer.
    Authors: Jaszczynska-Nowinka K, Rucinski M, Ziolkowska A, Markowska A, Malendowicz L
    Oncol Lett, 2014;7(5):1618-1624.
    Species: Human
    Sample Types: Plasma
  47. Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy.
    Authors: Zhang Y, Chen H, Sun C, Wang H, Liu M, Li Y, Nie X, Du M, Li D, Zhang J
    PLoS ONE, 2014;9(6):e99612.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Surgical sutures filled with adipose-derived stem cells promote wound healing.
    Authors: Reckhenrich A, Kirsch B, Wahl E, Schenck T, Rezaeian F, Harder Y, Foehr P, Machens H, Egana J
    PLoS ONE, 2014;9(3):e91169.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease.
    Authors: Krautkramer E, Grouls S, Hettwer D, Rafat N, Tonshoff B, Zeier M
    J Virol, 2014;88(1):483-9.
    Species: Human
    Sample Types: Plasma
  50. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Types: Serum
  51. Altered features and increased chemosensitivity of human breast cancer cells mediated by adipose tissue-derived mesenchymal stromal cells.
    Authors: Kucerova L, Skolekova S, Matuskova M, Bohac M, Kozovska Z
    BMC Cancer, 2013;13(0):535.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Plasma stromal cell-derived factor 1alpha/CXCL12 level predicts long-term adverse cardiovascular outcomes in patients with coronary artery disease.
    Authors: Ghasemzadeh N, Hritani A, De Staercke C, Eapen D, Veledar E, Al Kassem H, Khayata M, Zafari A, Sperling L, Hooper C, Vaccarino V, Mavromatis K, Quyyumi A
    Atherosclerosis, 2013;238(1):113-8.
  53. Intranasal delivery of mesenchymal stem cells significantly extends survival of irradiated mice with experimental brain tumors.
    Authors: Balyasnikova I, Prasol M, Ferguson S, Han Y, Ahmed A, Gutova M, Tobias A, Mustafi D, Rincon E, Zhang L, Aboody K, Lesniak M
    Mol Ther, 2013;22(1):140-8.
    Species: Human
    Sample Types: Cell Lysates
  54. Study of the quantitative, functional, cytogenetic, and immunoregulatory properties of bone marrow mesenchymal stem cells in patients with B-cell chronic lymphocytic leukemia.
    Authors: Pontikoglou C, Kastrinaki M, Klaus M, Kalpadakis C, Katonis P, Alpantaki K, Pangalis G, Papadaki H
    Stem Cells Dev, 2013;22(9):1329-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Differences are evident within the CXCR4-CXCL12 axis between ethnically divergent South African populations.
    Authors: Shalekoff S, Schramm D, Lassauniere R, Picton A, Tiemessen C
    Cytokine, 2013;61(3):792-800.
    Species: Human
    Sample Types: Plasma
  56. Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
    Authors: Lund TC, Stadem PS, Panoskaltsis-Mortari A, Raymond G, Miller WP, Tolar J, Orchard PJ
    PLoS ONE, 2012;7(2):e32218.
    Species: Human
    Sample Types: Serum
  57. Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
    Authors: Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N
    PLoS ONE, 2012;7(8):e43665.
    Species: Human
    Sample Types: Tissue Homogenates
  58. BMP9 regulates endoglin-dependent chemokine responses in endothelial cells.
    Authors: Young, Kira, Conley, Barbara, Romero, Diana, Tweedie, Eric, O'Neill, Christin, Pinz, Ilka, Brogan, Louise, Lindner, Volkhard, Liaw, Lucy, Vary, Calvin P
    Blood, 2012;120(20):4263-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
    Authors: Quesnel C, Piednoir P, Gelly J, Nardelli L, Garnier M, Lecon V, Lasocki S, Bouadma L, Philip I, Elbim C, Mentre F, Soler P, Crestani B, Dehoux M
    Crit. Care Med., 2012;40(1):21-8.
    Species: Human
    Sample Types: BALF
  60. Breast milk from Tanzanian women has divergent effects on cell-free and cell-associated HIV-1 infection in vitro.
    Authors: Lyimo M, Mosi M, Housman M, Zain-Ul-Abideen M, Lee F, Howell A, Connor R
    PLoS ONE, 2012;7(8):e43815.
    Species: Human
    Sample Types: Milk
  61. A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
    Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
    Cancer Res., 2012;72(1):66-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
    Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
    Obesity (Silver Spring), 2011;19(11):2130-6.
    Species: Human
    Sample Types: Plasma
  63. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2011;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Phase I and pharmacokinetic study of sunitinib in pediatric patients with refractory solid tumors: a children's oncology group study.
    Authors: Dubois SG, Shusterman S, Ingle AM, Ahern CH, Reid JM, Wu B, Baruchel S, Glade-Bender J, Ivy P, Grier HE, Adamson PC, Blaney SM
    Clin. Cancer Res., 2011;17(15):5113-22.
    Species: Human
    Sample Types: Plasma
  65. Levels of circulating CD45(dim)CD34(+)VEGFR2(+) progenitor cells correlate with outcome in metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors.
    Authors: Farace F, Gross-Goupil M, Tournay E, Taylor M, Vimond N, Jacques N, Billiot F, Mauguen A, Hill C, Escudier B
    Br. J. Cancer, 2011;104(7):1144-50.
    Species: Human
    Sample Types: Plasma
  66. Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
    Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
    Blood, 2011;117(13):3485-93.
    Species: Human
    Sample Types: Plasma
  67. Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.
    Authors: Gyongyosi M, Posa A, Pavo N, Hemetsberger R, Kvakan H, Steiner-Boker S, Petrasi Z, Manczur F, Pavo IJ, Edes IF, Wojta J, Glogar D, Huber K
    Thromb. Haemost., 2010;104(2):376-84.
    Species: Human
    Sample Types: Plasma
  68. Glioblastoma and endothelial cells cross-talk, mediated by SDF-1, enhances tumour invasion and endothelial proliferation by increasing expression of cathepsins B, S, and MMP-9.
    Authors: Kenig S, Alonso MB, Mueller MM, Lah TT
    Cancer Lett., 2010;289(1):53-61.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex.
    Authors: Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ, Kucia M, Janowska-Wieczorek A, Ratajczak J
    Leukemia, 2010;24(5):976-85.
    Species: Human
    Sample Types: Plasma
  70. Distraction osteogenesis induces endothelial progenitor cell mobilization without inflammatory response in man.
    Authors: Lee DY, Cho TJ, Lee HR, Park MS, Yoo WJ, Chung CY, Choi IH
    Bone, 2010;46(3):673-9.
    Species: Human
    Sample Types: Plasma
  71. Stromal cell-derived factor-1 is essential for photoreceptor cell protection in retinal detachment.
    Authors: Otsuka H, Arimura N, Sonoda S
    Am. J. Pathol., 2010;177(5):2268-77.
    Species: Human
    Sample Types: Vitreous Humor
  72. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Dysregulation of stromal derived factor 1/CXCR4 axis in the megakaryocytic lineage in essential thrombocythemia.
    Authors: Salim JP, Goette NP, Lev PR, Chazarreta CD, Heller PG, Alvarez C, Molinas FC, Marta RF
    Br. J. Haematol., 2009;144(1):69-77.
    Species: Human
    Sample Types: Plasma
  74. Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study.
    Authors: Zhu AX, Sahani DV, Duda DG, di Tomaso E, Ancukiewicz M, Catalano OA, Sindhwani V, Blaszkowsky LS, Yoon SS, Lahdenranta J, Bhargava P, Meyerhardt J, Clark JW, Kwak EL, Hezel AF, Miksad R, Abrams TA, Enzinger PC, Fuchs CS, Ryan DP, Jain RK
    J. Clin. Oncol., 2009;27(18):3027-35.
    Species: Human
    Sample Types: Plasma
  75. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009;69(15):6249-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Types: Serum
  77. Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target.
    Authors: Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC
    Cancer Cell, 2009;16(4):309-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs.
    Authors: Makam M, Diaz D, Laval J, Gernez Y, Conrad CK, Dunn CE, Davies ZA, Moss RB, Herzenberg LA, Herzenberg LA, Tirouvanziam R
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5779-83.
    Species: Human
    Sample Types: Plasma
  79. Targeted disruption of the CXCL12/CXCR4 axis inhibits osteolysis in a murine model of myeloma-associated bone loss.
    Authors: Diamond P, Labrinidis A, Martin SK, Farrugia AN, Gronthos S, To LB, Fujii N, O'Loughlin PD, Evdokiou A, Zannettino AC
    J. Bone Miner. Res., 2009;24(7):1150-61.
    Species: Human
    Sample Types: Plasma
  80. Nodal lymphangiogenesis and metastasis: Role of tumor-induced lymphatic vessel activation in extramammary Paget's disease.
    Authors: Hirakawa S, Detmar M, Kerjaschki D, Nagamatsu S, Matsuo K, Tanemura A, Kamata N, Higashikawa K, Okazaki H, Kameda K, Nishida-Fukuda H, Mori H, Hanakawa Y, Sayama K, Shirakata Y, Tohyama M, Tokumaru S, Katayama I, Hashimoto K
    Am. J. Pathol., 2009;175(5):2235-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Tissue inhibitor of metalloproteinase-3 via oncolytic herpesvirus inhibits tumor growth and vascular progenitors.
    Authors: Mahller YY, Vaikunth SS, Ripberger MC, Baird WH, Saeki Y, Cancelas JA, Crombleholme TM, Cripe TP
    Cancer Res., 2008;68(4):1170-9.
    Species: Human
    Sample Types: Plasma
  82. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008;14(3):263-73.
  84. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: BALF
  85. Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium.
    Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW
    Cancer Res., 2007;67(9):4244-53.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells.
    Authors: Irhimeh MR, Fitton JH, Lowenthal RM
    Exp. Hematol., 2007;35(6):989-94.
  87. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
    Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
    Biochem. Biophys. Res. Commun., 2007;363(3):472-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007;25(29):4542-9.
    Species: Human
    Sample Types: Plasma
  89. Role of the CXCL12/CXCR4 axis in peritoneal carcinomatosis of gastric cancer.
    Authors: Yasumoto K, Koizumi K, Kawashima A, Saitoh Y, Arita Y, Shinohara K, Minami T, Nakayama T, Sakurai H, Takahashi Y, Yoshie O, Saiki I
    Cancer Res., 2006;66(4):2181-7.
    Species: Human
    Sample Types: Ascites Fluid
  90. Serum levels of syndecan-1 in B-cell chronic lymphocytic leukemia: correlation with the extent of angiogenesis and disease-progression risk in early disease.
    Authors: Molica S, Vitelli G, Mirabelli R, Digiesu G, Giannarelli D, Cuneo A, Ribatti D, Vacca A
    Leuk. Lymphoma, 2006;47(6):1034-40.
    Species: Human
    Sample Types: Serum
  91. Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
    Authors: Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H
    Cardiovasc. Res., 2006;71(3):455-65.
    Species: Rabbit
    Sample Types: Serum
  92. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  93. SDF-1 is both necessary and sufficient to promote proliferative retinopathy.
    Authors: Butler JM, Guthrie SM, Koc M, Afzal A, Caballero S, Brooks HL, Mames RN, Segal MS, Grant MB, Scott EW
    J. Clin. Invest., 2005;115(1):86-93.
    Species: Human
    Sample Types: Vitreous Humor
  94. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005;23(1):203-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  95. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Types: CSF
  96. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004;199(9):1213-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction.
    Authors: Massa M, Rosti V, Ferrario M, Campanelli R, Ramajoli I, Rosso R, De Ferrari GM, Ferlini M, Goffredo L, Bertoletti A, Klersy C, Pecci A, Moratti R, Tavazzi L
    Blood, 2004;105(1):199-206.
    Species: Human
    Sample Types: Plasma
  98. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
    Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
    Eur. J. Immunol., 2004;34(4):1164-74.
    Species: Human
    Sample Types: Plasma
  99. Vitreous levels of vascular endothelial growth factor and stromal-derived factor 1 in patients with diabetic retinopathy and cystoid macular edema before and after intraocular injection of triamcinolone.
    Authors: Brooks HL, Caballero S, Newell CK, Steinmetz RL, Watson D, Segal MS, Harrison JK, Scott EW, Grant MB
    Arch. Ophthalmol., 2004;122(12):1801-7.
    Species: Human
    Sample Types: Vitreous Humor
  100. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: CSF
  101. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
    Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C
    Blood, 2002;99(3):792-9.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 03/12/2019
Sample Tested: Serum

Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
By Anonymous on 04/30/2018
Sample Tested: A549 human lung carcinoma cell line

Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
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Sample Tested: Pancreatic cancer cells