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Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

R&D Systems | Catalog # DSA00

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)

Sensitivity

47 pg/mL

Assay Range

156-10000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
View all CXCL12/SDF-1 alpha ELISA Kits »

Product Summary for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant SDF-1 alpha

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 491 1700 3420 544 1600 3150
Standard Deviation 19 58 115 73 150 257
CV% 3.9 3.4 3.4 13.4 9.4 8.2

Recovery for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-108
Platelet-poor EDTA Plasma (n=5) 97 90-107
Platelet-poor Heparin Plasma (n=5) 97 90-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL12/SDF-1 alpha ELISA Linearity

Scientific Data Images for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

Human CXCL12/SDF-1 alpha ELISA Standard Curve

Human CXCL12/SDF-1 alpha ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.

 

Alternate Names

SDF1 alpha, SDF1a

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline)

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 alpha Products

Product Documents for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit

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  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Adult brain
    Verified Customer | Posted 11/16/2025
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant, HMVEC human microvascular endothelial cells and Cell Lysates
    Verified Customer | Posted 01/30/2023
    Human CXCL12/SDF-1 alpha Quantikine ELISA Kit DSA00
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Ali Baity
    Sample Tested: HMVEC human microvascular endothelial cells
    Verified Customer | Posted 12/15/2022
    Human CXCL12/SDF-1 alpha Quantikine ELISA Kit DSA00
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/08/2022
    Could detect in plasma at 10-fold and detectable in some urine samples at low concentration at 2-fold. No detected urine matrix effect
    Human CXCL12/SDF-1 alpha Quantikine ELISA Kit DSA00
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 06/26/2020
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/13/2020
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media
    Verified Customer | Posted 01/12/2020
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 03/12/2019
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: A549 human lung carcinoma cell line
    Verified Customer | Posted 04/30/2018
  • Human CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Pancreatic cancer cells
    Verified Customer | Posted 04/17/2018

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Protocols

View specific protocols for Human CXCL12/SDF-1 alpha Quantikine ELISA Kit (DSA00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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