Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

  (28 citations)
(3 Reviews)
    
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Assay Procedure
Citations (28)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (25 uL)
  • Sensitivity
    3.97 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human BLC/BCA-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC48 , Quantikine Immunoassay Control Set 398 for Human BLC/BCA-1 - Please Inquire
Product Summary
The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 50.2 136 294 54 138 285
Standard Deviation 2.2 4.5 8 5.1 13.2 24.8
CV% 4.3 3.3 2.7 9.4 9.6 8.7

Recovery

The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 87-110
EDTA Plasma (n=4) 99 92-107
Heparin Plasma (n=4) 101 92-115
Serum (n=4) 99 86-108
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural BLC/BCA-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 CXCL13/BLC/BCA-1 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL13/BLC/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.
  • Entrez Gene IDs:
    10563 (Human); 55985 (Mouse)
  • Alternate Names:
    ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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Species
Sample Type
  1. HIV-specific Fc effector function early in infection predicts the development of broadly neutralizing antibodies
    Authors: SI Richardson, AW Chung, H Natarajan, B Mabvakure, NN Mkhize, N Garrett, S Abdool Kar, PL Moore, ME Ackerman, G Alter, L Morris
    PLoS Pathog., 2018;14(4):e1006987.
    Species: Human
    Sample Type: Plasma
  2. Elevated C-X-C motif ligand 13 and B-cell-activating factor levels in neuromyelitis optica during remission
    Authors: S Wang, T Yang, J Wan, Y Zhang, Y Fan
    Brain Behav, 2017;7(4):e00648.
    Species: Human
    Sample Type: Serum
  3. Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection
    Authors: RM Paris, LG Milagres, E Moysi, JF Okulicz, BK Agan, A Ganesan, C Petrovas, RA Koup
    Pathog Immun, 2017;2(1):66-88.
    Species: Human
    Sample Type: Plasma
  4. BAFF Index and CXCL13 levels in the cerebrospinal fluid associate respectively with intrathecal IgG synthesis and cortical atrophy in multiple sclerosis at clinical onset
    Authors: M Puthenpara, L Federle, S Miante, A Zito, E Toffanin, S Ruggero, M Ermani, S Pravato, D Poggiali, P Perini, F Rinaldi, P Gallo
    J Neuroinflammation, 2017;14(1):11.
    Species: Human
    Sample Type: Serum
  5. Intrathecal Th17- and B cell-associated cytokine and chemokine responses in relation to clinical outcome in Lyme neuroborreliosis: a large retrospective study
    Authors: P Gyllemark, P Forsberg, J Ernerudh, AJ Henningsso
    J Neuroinflammation, 2017;14(1):27.
    Species: Human
    Sample Type: Serum
  6. CXCL13 is a plasma biomarker of germinal center activity
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):2702-7.
    Species: Human
    Sample Type: Plasma
  7. Predictive Value of Cytokines and Immune Activation Biomarkers in AIDS-Related Non-Hodgkin Lymphoma Treated with Rituximab plus Infusional EPOCH (AMC-034 trial).
    Authors: Epeldegui M, Lee J, Martinez A, Widney D, Magpantay L, Regidor D, Mitsuyasu R, Sparano J, Ambinder R, Martinez-Maza O
    Clin Cancer Res, 2016;22(2):328-36.
    Species: Human
    Sample Type: Plasma
  8. Biomarkers of inflammation and axonal degeneration/damage in patients with newly diagnosed multiple sclerosis: contributions of the soluble CD163 CSF/serum ratio to a biomarker panel.
    Authors: Stilund M, Gjelstrup M, Petersen T, Moller H, Rasmussen P, Christensen T
    PLoS ONE, 2015;10(4):e0119681.
    Species: Human
    Sample Type: Serum
  9. HIV-1 single-stranded RNA induces CXCL13 secretion in human monocytes via TLR7 activation and plasmacytoid dendritic cell-derived type I IFN.
    Authors: Cohen K, Dugast A, Alter G, McElrath M, Stamatatos L
    J Immunol, 2015;194(6):2769-75.
    Species: Human
    Sample Type: Plasma
  10. Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis.
    Authors: Senel M, Tumani H, Lauda F, Presslauer S, Mojib-Yezdani R, Otto M, Brettschneider J
    PLoS ONE, 2014;9(4):e88680.
    Species: Human
    Sample Type: CSF
  11. CXCL13 and neopterin concentrations in cerebrospinal fluid of patients with Lyme neuroborreliosis and other diseases that cause neuroinflammation.
    Authors: Hytonen J, Kortela E, Waris M, Puustinen J, Salo J, Oksi J
    J Neuroinflammation, 2014;11(0):103.
    Species: Human
    Sample Type: CSF
  12. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Type: Serum
  13. Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
    Authors: Tiberti N, Hainard A, Lejon V, Courtioux B, Matovu E, Enyaru JC, Robin X, Turck N, Kristensson K, Ngoyi DM, Vatunga GM, Krishna S, Buscher P, Bisser S, Ndung'u JM, Sanchez JC
    PLoS ONE, 2012;7(7):e40909.
    Species: Human
    Sample Type: CSF
  14. The chemokine CXCL13 is a prognostic marker in clinically isolated syndrome (CIS).
    Authors: Brettschneider J, Czerwoniak A, Senel M
    PLoS ONE, 2010;5(8):e11986.
    Species: Human
    Sample Type: Serum
  15. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Type: Plasma
  16. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009;283(1):29-35.
    Species: Human
    Sample Type: Serum
  17. Potential novel biomarkers of disease activity in rheumatoid arthritis patients: CXCL13, CCL23, transforming growth factor alpha, tumor necrosis factor receptor superfamily member 9, and macrophage colony-stimulating factor.
    Authors: Rioja I, Hughes FJ, Sharp CH, Warnock LC, Montgomery DS, Akil M, Wilson AG, Binks MH, Dickson MC
    Arthritis Rheum., 2008;58(8):2257-67.
    Species: Human
    Sample Type: Plasma
  18. Lymphoid chemokines in chronic neuroinflammation.
    Authors: Aloisi F, Columba-Cabezas S, Franciotta D, Rosicarelli B, Magliozzi R, Reynolds R, Ambrosini E, Coccia E, Salvetti M, Serafini B
    J. Neuroimmunol., 2008;198(1):106-12.
    Species: Human
    Sample Type: CSF
  19. Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
    Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
    Br. J. Cancer, 2008;99(6):930-8.
    Species: Human
    Sample Type: Serum
  20. Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis.
    Authors: Sansonno D, Tucci FA, Troiani L, Lauletta G, Montrone M, Conteduca V, Sansonno L, Dammacco F
    Blood, 2008;112(5):1620-7.
    Species: Human
    Sample Type: Serum
  21. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
    Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
    Blood, 2008;112(12):4401-10.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
    Authors: Lim HW, Kim CH
    J. Immunol., 2007;179(11):7448-56.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. High levels of inflammatory chemokines and cytokines in joint fluid and synovial tissue throughout the course of antibiotic-refractory lyme arthritis.
    Authors: Shin JJ, Glickstein LJ, Steere AC
    Arthritis Rheum., 2007;56(4):1325-35.
    Species: Human
    Sample Type: Synovial Fluid
  24. Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia.
    Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA
    Blood, 2007;110(9):3316-25.
    Species: Human
    Sample Type: Serum
  25. Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts.
    Authors: Munoz-Fernandez R, Blanco FJ, Frecha C, Martin F, Kimatrai M, Abadia-Molina AC, Garcia-Pacheco JM, Olivares EG
    J. Immunol., 2006;177(1):280-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Type: CSF
  28. IL1beta and TNFalpha differently modulate CXCL13 chemokine in stromal cells and osteoblasts isolated from osteoarthritis patients: evidence of changes associated to cell maturation.
    Authors: Lisignoli G, Cristino S, Toneguzzi S, Grassi F, Piacentini A, Cavallo C, Facchini A, Mariani E
    Exp. Gerontol., 2004;39(4):659-65.
    Species: Human
    Sample Type: Cell Culture Supernates
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