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Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

R&D Systems | Catalog # DCX130

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)

Sensitivity

3.97 pg/mL

Assay Range

7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
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Product Summary for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human BLC/BCA-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva - Samples from apparently healthy volunteers were evaluated for the presence of human BLC/BCA-1 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum* (n=35)81.939.4-25243.5
EDTA plasma (n=35)62.529.4-24642.7
Heparin plasma (n=35)63.521.7-24044.1
*Twenty-five additional serum samples were tested and values of 10.6 pg/mL, 379 pg/mL, and 638 pg/mL were observed in three samples. The remaining samples were consistent with the range listed above.

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Saliva (n=10)67.470ND-284
ND=Non-Detectable

Cell Culture Supernates:
Human peripheral blood cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernate were removed and assayed for levels of human BLC/BCA-1.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
UnstimulatedNDND
Stimulated11640
ND=Non-detectable

THP-1 human acute monocytic leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, stimulated with 1.0 μg/mL of recombinant human IFN-gamma for eight hours, and then stimulated with 1.0 μg/mL of LPS overnight. An aliquot was removed, assayed for detectable human BLC/BCA-1, and measured 15,922 pg/mL.





Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 50.2 136 294 54.0 138 285
Standard Deviation 2.2 4.5 8.0 5.1 13.2 24.8
CV% 4.3 3.3 2.7 9.4 9.6 8.7

Recovery for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 87-110
EDTA Plasma (n=4) 99 92-107
Heparin Plasma (n=4) 101 92-115
Serum (n=4) 99 86-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of natural BLC/BCA-1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL13/BLC/BCA-1 ELISA Linearity

Scientific Data Images for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Human CXCL13/BLC/BCA-1 ELISA Standard Curve

Human CXCL13/BLC/BCA-1 ELISA Standard Curve

Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index (ESSDAI) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome (n = 58). The nine proteins were CXCL13, TNF receptor 2 (TNF-R2), CD48, B-cell activating factor (BAFF), programmed cell death protein 1 ligand 2 (PD-L2), lymphocyte activation gene-3 (LAG-3), beta 2-microglobulin ( beta 2MG), ephrin type-B receptor 2 (EPHB2), and CD163. Pearson’s correlation test was used. The correlation coefficients (r) and P values are shown in the table. P <0.05 was considered significant Image collected and cropped by CiteAb from the following open publication (https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-01…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL13/BLC/BCA-1

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell  homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naive B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer's patch development.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

Additional CXCL13/BLC/BCA-1 Products

Product Documents for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

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  • Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: LNCaP human prostate cancer cell line
    Verified Customer | Posted 06/07/2022
    The standard curve shows a good fit. Easy to use, and take ~5hours from start to get a result.
    Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit DCX130
  • Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/04/2020
  • Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
    Name: Chi P
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 04/23/2018
  • Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
    Name: Jessica Fernando
    Sample Tested: Serum
    Verified Customer | Posted 12/04/2017
  • Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit
    Name: Jessica Fernando
    Sample Tested: Serum
    Verified Customer | Posted 10/23/2017

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Protocols

View specific protocols for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit (DCX130):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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