Human CXCL14/BRAK Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL14/BRAK in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 131107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CXCL14/BRAK
    Ser35-Glu111
    Accession # O95715
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
    • Human CXCL14/BRAK Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human CXCL14/BRAK Antibody (Catalog # MAB866)
  • ELISA Detection (Matched Antibody Pair)
    0.5-2.0 µg/mL 
    Human CXCL14/BRAK Biotinylated Antibody (Catalog # BAM8661)
  • ELISA Standard
     
    Recombinant Human CXCL14/BRAK Protein, CF (Catalog # 866-CX)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL14/BRAK

CXCL14/BRAK (breast and kidney-expressed chemokine), also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of CXC chemokine superfamily (1 - 5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Human CXCL14 shares approximately 30% aa sequence identity with MIP-2 alpha (GRO beta ) as well as MIP-2 beta  (GRO gamma ). The gene for CXCL14 has been mapped human chromosome 5q31. Unlike the MIP-2 chemokines, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important for regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

  • References:
    1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
    2. Cao, X. et al. (2000) J. Immunol. 165:2588.
    3. Kurth, I. et al. (2001) J. Exp., Med. 194:855.
    4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
    5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.
  • Entrez Gene IDs:
    9547 (Human); 57266 (Mouse)
  • Alternate Names:
    BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14
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