Human CXCL4/PF4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF795
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human CXCL4/PF4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL4/PF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse PF4, recombinant human (rh) GRO alpha, and rhGRO beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CXCL4/PF4 (R&D Systems, Catalog # 795-P4)
Glu32-Ser101
Accession # P02776
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CXCL4/PF4 (Catalog # 795-P4)

Human CXCL4/PF4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CXCL4/PF4 Antibody (Catalog # MAB7951)

Standard: Recombinant Human CXCL4/PF4 Protein (Catalog # 795-P4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL4/PF4

CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References
  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Scheuerer, B. et al. (2000) Blood 95:1158.
  3. Perollet, C. et al. (1998) Blood 91:3289.
  4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
  5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
  6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Entrez Gene IDs
5196 (Human); 56744 (Mouse); 360918 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A

Product Datasheets

Citations for Human CXCL4/PF4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction
    Authors: JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch
    Pharmaceuticals (Basel), 2017;10(2):.
    Species: Human
    Sample Types: Platelet Rich Plasma (PRP)
    Applications: ELISA Development (Detection)
  2. Collagen can selectively trigger a platelet secretory phenotype via glycoprotein VI.
    Authors: Ollivier V, Syvannarath V, Gros A, Butt A, Loyau S, Jandrot-Perrus M, Ho-Tin-Noe B
    PLoS ONE, 2014;9(8):e104712.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study.
    Authors: Snoep JD, Roest M, Barendrecht AD
    J. Thromb. Haemost., 2010;8(5):906-13.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
    Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
    Nat. Med., 2009;15(1):97-103.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human CXCL4/PF4 Biotinylated Antibody

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Human CXCL4/PF4 Biotinylated Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in a sandwich ELISA with MAB7951 as the capture.