Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, Flow Cytometry, ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CXCL4/PF4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL4/PF4 (R&D Systems, Catalog # 795-P4)
Glu32-Ser101
Accession # P02776

Specificity

Detects human CXCL4/PF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse PF4, recombinant human (rh) GRO alpha, and rhGRO beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CXCL4/PF4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL4/PF4 (Catalog # 795-P4)

Human CXCL4/PF4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CXCL4/PF4 Antibody (Catalog # MAB7951)
  • Standard: Recombinant Human CXCL4/PF4 Protein (Catalog # 795-P4)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BAF795 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL4/PF4

CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References

  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Scheuerer, B. et al. (2000) Blood 95:1158.
  3. Perollet, C. et al. (1998) Blood 91:3289.
  4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
  5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
  6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

Alternate Names

PF4

Entrez Gene IDs

5196 (Human); 56744 (Mouse); 360918 (Rat)

Gene Symbol

PF4

UniProt

P02776

Additional CXCL4/PF4 Products

Product Documents for Human CXCL4/PF4 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL4/PF4 Biotinylated Antibody

For research use only

Citations for Human CXCL4/PF4 Biotinylated Antibody

Customer Reviews for Human CXCL4/PF4 Biotinylated Antibody (1)

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  • Human CXCL4/PF4 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/17/2017
    This antibody was used as the detection in a sandwich ELISA with MAB7951 as the capture.
    Human CXCL4/PF4 Biotinylated Antibody BAF795

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